Retinoic Acid and Estrogen Modulation of Insulin-like Growth Factor Binding Protein-4 Gene Expression and the Estrogen Receptor Status of Human Breast Carcinoma Cells

M. Saeed Sheikh, Zhi Ming Shao, Arif Hussain, Jian Chyi Chen, Charles T. Roberts, Derek Leroith, Joseph A. Fontana

    Research output: Contribution to journalArticle

    25 Scopus citations

    Abstract

    Retinoic acid (RA) and estrogen modulation of insulin like-growth factor binding protein-4 (IGFBP-4) gene expression was investigated in a number of human breast carcinoma (HBC) cell lines. Our results indicate that RA and/or estrogen modulation of IGFBP-4 mRNA levels appear to correlate with the ER-status of HBC cell lines. RA and estrogen enhanced IGFBP-4 mRNA levels in ER-positive MCF-7, RRO-I and ZR-75 cell lines and the effect of both of these agents in combination was additive. Although both individually also enhanced IGFBP-4 mRNA levels in ER-positive T47D cells, their effect in combination was antagonistic in this cell line. Transfection of human ER into ER-negative MDA-MB-231 cells conferred RA and estrogen the ability to enhance IGFBP-4 mRNA but both agents failed to concomitantly modulate IGFBP-4 levels in the CM suggesting a dual regulation by these agents at transcription/post-transcription and translation/secretion levels.

    Original languageEnglish (US)
    Pages (from-to)1232-1238
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume193
    Issue number3
    DOIs
    StatePublished - Jun 30 1993

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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