TY - JOUR
T1 - Retinal pigment epithelial cells produce interleukin-1β and granulocyte-macrophage colony-stimulating factor in response to interleukin-1α
AU - Planck, Stephen R.
AU - Huang, Xiao Na
AU - Robertson, Joseph E.
AU - Rosenbaum, James T.
N1 - Funding Information:
ACKNOWLEDGEMENTS This project was funded by NIH grants EY07373 and EY06484.
PY - 1993
Y1 - 1993
N2 - The retinal pigment epithelium (RPE) is clinically involved in diverse ocular inflammatory diseases. Because perturbed RPE cells produce a variety of inflammatory substances, RPE cells may play an integral part in these diseases. Interleukin-1 (IL-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) are pleiotropic cytokines with the ability to trigger numerous inflammatory responses. This report shows that cultured human RPE cells synthesize interleukin-1 β (IL-1β) and GM-CSF in response to the potentially inflammatory cytokine, IL-1α but not to E. coli endotoxin. Control RPE cells made little or no mRNA or protein for either IL-1β or GM-CSF. Upon stimulation of the cells by IL-1α both IL-1β and GM-CSF mRNAs were readily apparent by 3 hours, persisted for over 24 hours, and were translated into immunologically detectable proteins. GM-CSF protein was secreted into the culture medium, whereas IL-1β protein remained cell associated. The IL-1αinduced mRNA and protein production were inhibited by dexamethasone. These observations provide additional evidence that RPE cells are capable of playing a pivotal role during ocular inflammation.
AB - The retinal pigment epithelium (RPE) is clinically involved in diverse ocular inflammatory diseases. Because perturbed RPE cells produce a variety of inflammatory substances, RPE cells may play an integral part in these diseases. Interleukin-1 (IL-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) are pleiotropic cytokines with the ability to trigger numerous inflammatory responses. This report shows that cultured human RPE cells synthesize interleukin-1 β (IL-1β) and GM-CSF in response to the potentially inflammatory cytokine, IL-1α but not to E. coli endotoxin. Control RPE cells made little or no mRNA or protein for either IL-1β or GM-CSF. Upon stimulation of the cells by IL-1α both IL-1β and GM-CSF mRNAs were readily apparent by 3 hours, persisted for over 24 hours, and were translated into immunologically detectable proteins. GM-CSF protein was secreted into the culture medium, whereas IL-1β protein remained cell associated. The IL-1αinduced mRNA and protein production were inhibited by dexamethasone. These observations provide additional evidence that RPE cells are capable of playing a pivotal role during ocular inflammation.
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U2 - 10.3109/02713689308999465
DO - 10.3109/02713689308999465
M3 - Article
C2 - 8482109
AN - SCOPUS:0027215297
SN - 0271-3683
VL - 12
SP - 205
EP - 212
JO - Current Eye Research
JF - Current Eye Research
IS - 3
ER -