Retinal NMDA receptor function and expression are altered in a mouse lacking D-amino acid oxidase

Eric C. Gustafson, Catherine Morgans, Merve Tekmen, Steven J. Sullivan, Manuel Esguerra, Ryuichi Konno, Robert F. Miller

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    D-serine is present in the vertebrate retina and serves as a coagonist for the N-methyl-D-aspartate (NMDA) receptors of ganglion cells. Although the enzyme D-amino acid oxidase (DAO) has been implicated as a pathway for D-serine degradation, its role in the retina has not been established. In this study, we investigated the role of DAO in regulating D-serine levels using a mutant mouse line deficient in DAO (ddY/DAO-) and compared these results with their wild-type counterparts (ddY/DAO+). Our results show that DAO is functionally present in the mouse retina and normally serves to reduce the background levels of D-serine. The enzymatic activity of DAO was restricted to the inner plexiform layer as determined by histochemical analysis. Using capillary electrophoresis, we showed that mutant mice had much higher levels of D-serine. Whole cell recordings from identified retinal ganglion cells demonstrated that DAO-deficient animals had light-evoked synaptic activity strongly biased toward a high NMDA-to-AMPA receptor ratio. In contrast, recordings from wild-type ganglion cells showed a more balanced ratio between the two receptor subclasses. Immunostaining for AMPA and NMDA receptors was carried out to compare the two receptor ratios by quantitative immunofluorescence. These studies revealed that the mutant mouse had a significantly higher representation of NMDA receptors compared with the wild-type controls. We conclude that 1) DAO is an important regulatory enzyme and normally functions to reduce D-serine levels in the retina, and 2) D-serine levels play a role in the expression of NMDA receptors and the NMDA-to-AMPA receptor ratio.

    Original languageEnglish (US)
    Pages (from-to)2718-2726
    Number of pages9
    JournalJournal of Neurophysiology
    Volume110
    Issue number12
    DOIs
    StatePublished - Dec 15 2013

    Fingerprint

    D-Amino-Acid Oxidase
    N-Methyl-D-Aspartate Receptors
    Serine
    Retina
    AMPA Receptors
    N-Methylaspartate
    Ganglia
    alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
    Retinal Ganglion Cells
    Capillary Electrophoresis
    Patch-Clamp Techniques
    Enzymes
    Fluorescent Antibody Technique
    Vertebrates

    Keywords

    • D-amino acid oxidase
    • D-serine
    • Glutamate receptors
    • Mouse
    • Retinal ganglion cells

    ASJC Scopus subject areas

    • Physiology
    • Neuroscience(all)

    Cite this

    Gustafson, E. C., Morgans, C., Tekmen, M., Sullivan, S. J., Esguerra, M., Konno, R., & Miller, R. F. (2013). Retinal NMDA receptor function and expression are altered in a mouse lacking D-amino acid oxidase. Journal of Neurophysiology, 110(12), 2718-2726. https://doi.org/10.1152/jn.00310.2013

    Retinal NMDA receptor function and expression are altered in a mouse lacking D-amino acid oxidase. / Gustafson, Eric C.; Morgans, Catherine; Tekmen, Merve; Sullivan, Steven J.; Esguerra, Manuel; Konno, Ryuichi; Miller, Robert F.

    In: Journal of Neurophysiology, Vol. 110, No. 12, 15.12.2013, p. 2718-2726.

    Research output: Contribution to journalArticle

    Gustafson, EC, Morgans, C, Tekmen, M, Sullivan, SJ, Esguerra, M, Konno, R & Miller, RF 2013, 'Retinal NMDA receptor function and expression are altered in a mouse lacking D-amino acid oxidase', Journal of Neurophysiology, vol. 110, no. 12, pp. 2718-2726. https://doi.org/10.1152/jn.00310.2013
    Gustafson, Eric C. ; Morgans, Catherine ; Tekmen, Merve ; Sullivan, Steven J. ; Esguerra, Manuel ; Konno, Ryuichi ; Miller, Robert F. / Retinal NMDA receptor function and expression are altered in a mouse lacking D-amino acid oxidase. In: Journal of Neurophysiology. 2013 ; Vol. 110, No. 12. pp. 2718-2726.
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