Retinal blood flow response to hyperoxia measured with en face doppler optical coherence tomography

Alex D. Pechauer, Ou Tan, Liang Liu, Jia Yali, Vivian Hou, William Hills, David Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

PURPOSE. To use multiplane en face Doppler optical coherence tomography (OCT) to measure the change in total retinal blood flow (TRBF) in response to hyperoxia. METHODS. One eye of each healthy human participant (n = 8) was scanned with a commercial high-speed (70-kHz) spectral OCT system. Three repeated scans were captured at baseline and after 10 minutes of oxygen (hyperoxia) by open nasal mask. The procedure was performed twice on day 1 and once more on day 2. Blood flow of each vein was estimated using Doppler OCT at an optimized en face plane. The TRBF was summed from all veins at the optic disc. The TRBF hyperoxic response was calculated as the TRBF percent change from baseline. RESULTS. Participants experienced a 23.6% ± 10.7% (mean ± standard deviation [SD]) decrease (P <0.001, paired t-test) in TRBF during hyperoxia. The within-day repeatability of baseline TRBF was 4.1% and the between-day reproducibility was 10.9% coefficient of variation (CV). Between-grader reproducibility was 3.9% CV. The repeatability and reproducibility (pooled SD) of hyperoxic response were 6.1% and 6.4%, respectively. CONCLUSIONS. The multiplane en face Doppler OCT algorithm was able to detect, in all participants, a decreased TRBF in response to hyperoxia. The response magnitude for each participant varied among repeated trials, and the averaging of multiple trials was helpful in establishing the individual response. This technique shows good potential for the clinical investigation of vascular autoregulation.

Original languageEnglish (US)
Pages (from-to)OCT141-OCT145
JournalInvestigative Ophthalmology and Visual Science
Volume57
Issue number9
DOIs
StatePublished - Jul 1 2016

Fingerprint

Hyperoxia
Optical Coherence Tomography
Veins
Optic Disk
Masks
Nose
Blood Vessels
Healthy Volunteers
Homeostasis
Oxygen

Keywords

  • En face doppler optical coherence tomography
  • Hyperoxia
  • Total retinal blood flow

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

@article{b2704983976d47099bd3136de6b920a2,
title = "Retinal blood flow response to hyperoxia measured with en face doppler optical coherence tomography",
abstract = "PURPOSE. To use multiplane en face Doppler optical coherence tomography (OCT) to measure the change in total retinal blood flow (TRBF) in response to hyperoxia. METHODS. One eye of each healthy human participant (n = 8) was scanned with a commercial high-speed (70-kHz) spectral OCT system. Three repeated scans were captured at baseline and after 10 minutes of oxygen (hyperoxia) by open nasal mask. The procedure was performed twice on day 1 and once more on day 2. Blood flow of each vein was estimated using Doppler OCT at an optimized en face plane. The TRBF was summed from all veins at the optic disc. The TRBF hyperoxic response was calculated as the TRBF percent change from baseline. RESULTS. Participants experienced a 23.6{\%} ± 10.7{\%} (mean ± standard deviation [SD]) decrease (P <0.001, paired t-test) in TRBF during hyperoxia. The within-day repeatability of baseline TRBF was 4.1{\%} and the between-day reproducibility was 10.9{\%} coefficient of variation (CV). Between-grader reproducibility was 3.9{\%} CV. The repeatability and reproducibility (pooled SD) of hyperoxic response were 6.1{\%} and 6.4{\%}, respectively. CONCLUSIONS. The multiplane en face Doppler OCT algorithm was able to detect, in all participants, a decreased TRBF in response to hyperoxia. The response magnitude for each participant varied among repeated trials, and the averaging of multiple trials was helpful in establishing the individual response. This technique shows good potential for the clinical investigation of vascular autoregulation.",
keywords = "En face doppler optical coherence tomography, Hyperoxia, Total retinal blood flow",
author = "Pechauer, {Alex D.} and Ou Tan and Liang Liu and Jia Yali and Vivian Hou and William Hills and David Huang",
year = "2016",
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doi = "10.1167/iovs.15-18917",
language = "English (US)",
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TY - JOUR

T1 - Retinal blood flow response to hyperoxia measured with en face doppler optical coherence tomography

AU - Pechauer, Alex D.

AU - Tan, Ou

AU - Liu, Liang

AU - Yali, Jia

AU - Hou, Vivian

AU - Hills, William

AU - Huang, David

PY - 2016/7/1

Y1 - 2016/7/1

N2 - PURPOSE. To use multiplane en face Doppler optical coherence tomography (OCT) to measure the change in total retinal blood flow (TRBF) in response to hyperoxia. METHODS. One eye of each healthy human participant (n = 8) was scanned with a commercial high-speed (70-kHz) spectral OCT system. Three repeated scans were captured at baseline and after 10 minutes of oxygen (hyperoxia) by open nasal mask. The procedure was performed twice on day 1 and once more on day 2. Blood flow of each vein was estimated using Doppler OCT at an optimized en face plane. The TRBF was summed from all veins at the optic disc. The TRBF hyperoxic response was calculated as the TRBF percent change from baseline. RESULTS. Participants experienced a 23.6% ± 10.7% (mean ± standard deviation [SD]) decrease (P <0.001, paired t-test) in TRBF during hyperoxia. The within-day repeatability of baseline TRBF was 4.1% and the between-day reproducibility was 10.9% coefficient of variation (CV). Between-grader reproducibility was 3.9% CV. The repeatability and reproducibility (pooled SD) of hyperoxic response were 6.1% and 6.4%, respectively. CONCLUSIONS. The multiplane en face Doppler OCT algorithm was able to detect, in all participants, a decreased TRBF in response to hyperoxia. The response magnitude for each participant varied among repeated trials, and the averaging of multiple trials was helpful in establishing the individual response. This technique shows good potential for the clinical investigation of vascular autoregulation.

AB - PURPOSE. To use multiplane en face Doppler optical coherence tomography (OCT) to measure the change in total retinal blood flow (TRBF) in response to hyperoxia. METHODS. One eye of each healthy human participant (n = 8) was scanned with a commercial high-speed (70-kHz) spectral OCT system. Three repeated scans were captured at baseline and after 10 minutes of oxygen (hyperoxia) by open nasal mask. The procedure was performed twice on day 1 and once more on day 2. Blood flow of each vein was estimated using Doppler OCT at an optimized en face plane. The TRBF was summed from all veins at the optic disc. The TRBF hyperoxic response was calculated as the TRBF percent change from baseline. RESULTS. Participants experienced a 23.6% ± 10.7% (mean ± standard deviation [SD]) decrease (P <0.001, paired t-test) in TRBF during hyperoxia. The within-day repeatability of baseline TRBF was 4.1% and the between-day reproducibility was 10.9% coefficient of variation (CV). Between-grader reproducibility was 3.9% CV. The repeatability and reproducibility (pooled SD) of hyperoxic response were 6.1% and 6.4%, respectively. CONCLUSIONS. The multiplane en face Doppler OCT algorithm was able to detect, in all participants, a decreased TRBF in response to hyperoxia. The response magnitude for each participant varied among repeated trials, and the averaging of multiple trials was helpful in establishing the individual response. This technique shows good potential for the clinical investigation of vascular autoregulation.

KW - En face doppler optical coherence tomography

KW - Hyperoxia

KW - Total retinal blood flow

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U2 - 10.1167/iovs.15-18917

DO - 10.1167/iovs.15-18917

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