Retention of nociceptor responses during deep barbiturate anesthesia in frogs

Hall Downes, Dennis Koop, Bethany Klopfenstein, Nickola Lessov

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Bullfrogs (Rana catesbeiana) anesthetized with a large dose of thiopental (42.8 mg/kg) retained movement responses to nociceptor stimuli despite an average plasma drug level of 51 mg/l, of which 63% was bound to plasma proteins. This concentration, when corrected to include only unbound and uncharged drug, was 2-fold greater than those reported to abolish nociceptor response (NR) during surgical anesthesia in man. The median anesthetic dose (AD50) for loss of the righting reflex was 11.2 mg/kg by s.c. injection into the abdominal lymph sac; however, at 54.0 mg/kg, all frogs retained NRs, although otherwise deeply anesthetized. The ratio of NR-blocking dose to light AD was thus >4.8, as compared to

Original languageEnglish (US)
Pages (from-to)203-210
Number of pages8
JournalComparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology
Volume124
Issue number2
DOIs
StatePublished - Oct 1999

Fingerprint

Nociceptors
Anura
Rana catesbeiana
Anesthesia
Righting Reflex
Thiopental
Lymph
Pharmaceutical Preparations
Anesthetics
Blood Proteins
Light
Injections
barbituric acid

Keywords

  • Analgesia
  • Anesthesia
  • Barbiturate
  • Blood levels
  • Frog
  • Nociception
  • Protein binding
  • Rana catesbeiana
  • Thiopental

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

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abstract = "Bullfrogs (Rana catesbeiana) anesthetized with a large dose of thiopental (42.8 mg/kg) retained movement responses to nociceptor stimuli despite an average plasma drug level of 51 mg/l, of which 63{\%} was bound to plasma proteins. This concentration, when corrected to include only unbound and uncharged drug, was 2-fold greater than those reported to abolish nociceptor response (NR) during surgical anesthesia in man. The median anesthetic dose (AD50) for loss of the righting reflex was 11.2 mg/kg by s.c. injection into the abdominal lymph sac; however, at 54.0 mg/kg, all frogs retained NRs, although otherwise deeply anesthetized. The ratio of NR-blocking dose to light AD was thus >4.8, as compared to",
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AU - Downes, Hall

AU - Koop, Dennis

AU - Klopfenstein, Bethany

AU - Lessov, Nickola

PY - 1999/10

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N2 - Bullfrogs (Rana catesbeiana) anesthetized with a large dose of thiopental (42.8 mg/kg) retained movement responses to nociceptor stimuli despite an average plasma drug level of 51 mg/l, of which 63% was bound to plasma proteins. This concentration, when corrected to include only unbound and uncharged drug, was 2-fold greater than those reported to abolish nociceptor response (NR) during surgical anesthesia in man. The median anesthetic dose (AD50) for loss of the righting reflex was 11.2 mg/kg by s.c. injection into the abdominal lymph sac; however, at 54.0 mg/kg, all frogs retained NRs, although otherwise deeply anesthetized. The ratio of NR-blocking dose to light AD was thus >4.8, as compared to

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