Resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells

Jason D. Kenealey, Lalita Subramanian, Paul R. Van Ginkel, Soesiawati Darjatmoko, Mary J. Lindstrom, Veronika Somoza, Sunil K. Ghosh, Zhenlei Song, Richard P. Hsung, Glen S. Kwon, Kevin W. Eliceiri, Daniel Albert, Arthur S. Polans

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Resveratrol, a nontoxic polyphenol, has been shown to inhibit tumor growth in a xenograft mouse model of neuroblasoma. However, resveratrol is rapidly metabolized, mainly to its glucuronidated and sulfated derivatives. This study demonstrates that resveratrol alone, and not the glucuronidated or sulfated metabolites, is taken up into tumor cells, induces a rise in [Ca 2+ ]i, and ultimately leads to a decrease in tumor cell viability. A new water-soluble resveratrol formulation was delivered directly at the site of the tumor in a neuroblastoma mouse model. The amount of unmodified resveratrol associated with the tumor increased more than 1000-fold. The increase of unmodified resveratrol associated with the tumor resulted in tumor regression. The number of residual tumor cells that remained viable also decreased as the ratio of the metabolites relative to unmodified resveratrol declined.

Original languageEnglish (US)
Pages (from-to)4979-4986
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number9
DOIs
StatePublished - May 11 2011
Externally publishedYes

Fingerprint

resveratrol
Metabolites
Neuroblastoma
Tumors
metabolites
Neoplasms
cells
neoplasms
Cells
animal models
Residual Neoplasm
remission
Polyphenols
Heterografts
growth models
cell viability
Cell Survival
polyphenols
Derivatives
Water

Keywords

  • Bioavailability
  • Calcium signaling
  • Neuroblastoma
  • Resveratrol

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kenealey, J. D., Subramanian, L., Van Ginkel, P. R., Darjatmoko, S., Lindstrom, M. J., Somoza, V., ... Polans, A. S. (2011). Resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells. Journal of Agricultural and Food Chemistry, 59(9), 4979-4986. https://doi.org/10.1021/jf104901g

Resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells. / Kenealey, Jason D.; Subramanian, Lalita; Van Ginkel, Paul R.; Darjatmoko, Soesiawati; Lindstrom, Mary J.; Somoza, Veronika; Ghosh, Sunil K.; Song, Zhenlei; Hsung, Richard P.; Kwon, Glen S.; Eliceiri, Kevin W.; Albert, Daniel; Polans, Arthur S.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 9, 11.05.2011, p. 4979-4986.

Research output: Contribution to journalArticle

Kenealey, JD, Subramanian, L, Van Ginkel, PR, Darjatmoko, S, Lindstrom, MJ, Somoza, V, Ghosh, SK, Song, Z, Hsung, RP, Kwon, GS, Eliceiri, KW, Albert, D & Polans, AS 2011, 'Resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells', Journal of Agricultural and Food Chemistry, vol. 59, no. 9, pp. 4979-4986. https://doi.org/10.1021/jf104901g
Kenealey JD, Subramanian L, Van Ginkel PR, Darjatmoko S, Lindstrom MJ, Somoza V et al. Resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells. Journal of Agricultural and Food Chemistry. 2011 May 11;59(9):4979-4986. https://doi.org/10.1021/jf104901g
Kenealey, Jason D. ; Subramanian, Lalita ; Van Ginkel, Paul R. ; Darjatmoko, Soesiawati ; Lindstrom, Mary J. ; Somoza, Veronika ; Ghosh, Sunil K. ; Song, Zhenlei ; Hsung, Richard P. ; Kwon, Glen S. ; Eliceiri, Kevin W. ; Albert, Daniel ; Polans, Arthur S. / Resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells. In: Journal of Agricultural and Food Chemistry. 2011 ; Vol. 59, No. 9. pp. 4979-4986.
@article{fefd27ba5bea4e29b1422420bc9364aa,
title = "Resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells",
abstract = "Resveratrol, a nontoxic polyphenol, has been shown to inhibit tumor growth in a xenograft mouse model of neuroblasoma. However, resveratrol is rapidly metabolized, mainly to its glucuronidated and sulfated derivatives. This study demonstrates that resveratrol alone, and not the glucuronidated or sulfated metabolites, is taken up into tumor cells, induces a rise in [Ca 2+ ]i, and ultimately leads to a decrease in tumor cell viability. A new water-soluble resveratrol formulation was delivered directly at the site of the tumor in a neuroblastoma mouse model. The amount of unmodified resveratrol associated with the tumor increased more than 1000-fold. The increase of unmodified resveratrol associated with the tumor resulted in tumor regression. The number of residual tumor cells that remained viable also decreased as the ratio of the metabolites relative to unmodified resveratrol declined.",
keywords = "Bioavailability, Calcium signaling, Neuroblastoma, Resveratrol",
author = "Kenealey, {Jason D.} and Lalita Subramanian and {Van Ginkel}, {Paul R.} and Soesiawati Darjatmoko and Lindstrom, {Mary J.} and Veronika Somoza and Ghosh, {Sunil K.} and Zhenlei Song and Hsung, {Richard P.} and Kwon, {Glen S.} and Eliceiri, {Kevin W.} and Daniel Albert and Polans, {Arthur S.}",
year = "2011",
month = "5",
day = "11",
doi = "10.1021/jf104901g",
language = "English (US)",
volume = "59",
pages = "4979--4986",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Resveratrol metabolites do not elicit early pro-apoptotic mechanisms in neuroblastoma cells

AU - Kenealey, Jason D.

AU - Subramanian, Lalita

AU - Van Ginkel, Paul R.

AU - Darjatmoko, Soesiawati

AU - Lindstrom, Mary J.

AU - Somoza, Veronika

AU - Ghosh, Sunil K.

AU - Song, Zhenlei

AU - Hsung, Richard P.

AU - Kwon, Glen S.

AU - Eliceiri, Kevin W.

AU - Albert, Daniel

AU - Polans, Arthur S.

PY - 2011/5/11

Y1 - 2011/5/11

N2 - Resveratrol, a nontoxic polyphenol, has been shown to inhibit tumor growth in a xenograft mouse model of neuroblasoma. However, resveratrol is rapidly metabolized, mainly to its glucuronidated and sulfated derivatives. This study demonstrates that resveratrol alone, and not the glucuronidated or sulfated metabolites, is taken up into tumor cells, induces a rise in [Ca 2+ ]i, and ultimately leads to a decrease in tumor cell viability. A new water-soluble resveratrol formulation was delivered directly at the site of the tumor in a neuroblastoma mouse model. The amount of unmodified resveratrol associated with the tumor increased more than 1000-fold. The increase of unmodified resveratrol associated with the tumor resulted in tumor regression. The number of residual tumor cells that remained viable also decreased as the ratio of the metabolites relative to unmodified resveratrol declined.

AB - Resveratrol, a nontoxic polyphenol, has been shown to inhibit tumor growth in a xenograft mouse model of neuroblasoma. However, resveratrol is rapidly metabolized, mainly to its glucuronidated and sulfated derivatives. This study demonstrates that resveratrol alone, and not the glucuronidated or sulfated metabolites, is taken up into tumor cells, induces a rise in [Ca 2+ ]i, and ultimately leads to a decrease in tumor cell viability. A new water-soluble resveratrol formulation was delivered directly at the site of the tumor in a neuroblastoma mouse model. The amount of unmodified resveratrol associated with the tumor increased more than 1000-fold. The increase of unmodified resveratrol associated with the tumor resulted in tumor regression. The number of residual tumor cells that remained viable also decreased as the ratio of the metabolites relative to unmodified resveratrol declined.

KW - Bioavailability

KW - Calcium signaling

KW - Neuroblastoma

KW - Resveratrol

UR - http://www.scopus.com/inward/record.url?scp=79955689719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955689719&partnerID=8YFLogxK

U2 - 10.1021/jf104901g

DO - 10.1021/jf104901g

M3 - Article

C2 - 21401048

AN - SCOPUS:79955689719

VL - 59

SP - 4979

EP - 4986

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 9

ER -