Restraint stress slows cutaneous wound healing in mice

David A. Padgett, Phillip Marucha, John F. Sheridan

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

The impact of stress on cutaneous wound healing was assessed in a murine model. Female, hairless SKH-1 mice, 6-8 weeks of age were subjected to restraint stress (RST) 3 days before and for 5 days following dorsal application of a 3.5-mm sterile punch wound. Control mice were wounded, but not restrained. Using photography and image analysis, the rate of wound healing was compared between the two groups. Wounds on control mice healed on average 3.10 days sooner than RST-treated mice. In addition, cross-sectional, morphometric analysis of the dermal and epidermal layers revealed reduced inflammation surrounding wounds from RST mice at 1, 3, and 5 days after wounding. In the RST group, serum corticosterone levels averaged 162.5 ng/ml compared to 35.7 ng/ml in the controls. Treatment of RST-stressed animals with the glucocorticoid receptor antagonist RU40555 resulted in healing rates comparable to those of control animals. Thus, the reduction in inflammation and delayed healing correlated with serum corticosterone levels and suggest that disruption of neuroendocrine homeostasis modulates wound healing.

Original languageEnglish (US)
Pages (from-to)64-73
Number of pages10
JournalBrain, Behavior, and Immunity
Volume12
Issue number1
DOIs
StatePublished - Mar 1998
Externally publishedYes

Fingerprint

Wound Healing
Skin
Corticosterone
Wounds and Injuries
Inflammation
Photography
Glucocorticoid Receptors
Serum
Homeostasis
Cross-Sectional Studies

Keywords

  • Glucocorticoid
  • Mice
  • RU40555
  • Stress
  • Wound healing

ASJC Scopus subject areas

  • Immunology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Restraint stress slows cutaneous wound healing in mice. / Padgett, David A.; Marucha, Phillip; Sheridan, John F.

In: Brain, Behavior, and Immunity, Vol. 12, No. 1, 03.1998, p. 64-73.

Research output: Contribution to journalArticle

Padgett, David A. ; Marucha, Phillip ; Sheridan, John F. / Restraint stress slows cutaneous wound healing in mice. In: Brain, Behavior, and Immunity. 1998 ; Vol. 12, No. 1. pp. 64-73.
@article{1a7aa752cca24709ae766f92979cdaf5,
title = "Restraint stress slows cutaneous wound healing in mice",
abstract = "The impact of stress on cutaneous wound healing was assessed in a murine model. Female, hairless SKH-1 mice, 6-8 weeks of age were subjected to restraint stress (RST) 3 days before and for 5 days following dorsal application of a 3.5-mm sterile punch wound. Control mice were wounded, but not restrained. Using photography and image analysis, the rate of wound healing was compared between the two groups. Wounds on control mice healed on average 3.10 days sooner than RST-treated mice. In addition, cross-sectional, morphometric analysis of the dermal and epidermal layers revealed reduced inflammation surrounding wounds from RST mice at 1, 3, and 5 days after wounding. In the RST group, serum corticosterone levels averaged 162.5 ng/ml compared to 35.7 ng/ml in the controls. Treatment of RST-stressed animals with the glucocorticoid receptor antagonist RU40555 resulted in healing rates comparable to those of control animals. Thus, the reduction in inflammation and delayed healing correlated with serum corticosterone levels and suggest that disruption of neuroendocrine homeostasis modulates wound healing.",
keywords = "Glucocorticoid, Mice, RU40555, Stress, Wound healing",
author = "Padgett, {David A.} and Phillip Marucha and Sheridan, {John F.}",
year = "1998",
month = "3",
doi = "10.1006/brbi.1997.0512",
language = "English (US)",
volume = "12",
pages = "64--73",
journal = "Brain, Behavior, and Immunity",
issn = "0889-1591",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Restraint stress slows cutaneous wound healing in mice

AU - Padgett, David A.

AU - Marucha, Phillip

AU - Sheridan, John F.

PY - 1998/3

Y1 - 1998/3

N2 - The impact of stress on cutaneous wound healing was assessed in a murine model. Female, hairless SKH-1 mice, 6-8 weeks of age were subjected to restraint stress (RST) 3 days before and for 5 days following dorsal application of a 3.5-mm sterile punch wound. Control mice were wounded, but not restrained. Using photography and image analysis, the rate of wound healing was compared between the two groups. Wounds on control mice healed on average 3.10 days sooner than RST-treated mice. In addition, cross-sectional, morphometric analysis of the dermal and epidermal layers revealed reduced inflammation surrounding wounds from RST mice at 1, 3, and 5 days after wounding. In the RST group, serum corticosterone levels averaged 162.5 ng/ml compared to 35.7 ng/ml in the controls. Treatment of RST-stressed animals with the glucocorticoid receptor antagonist RU40555 resulted in healing rates comparable to those of control animals. Thus, the reduction in inflammation and delayed healing correlated with serum corticosterone levels and suggest that disruption of neuroendocrine homeostasis modulates wound healing.

AB - The impact of stress on cutaneous wound healing was assessed in a murine model. Female, hairless SKH-1 mice, 6-8 weeks of age were subjected to restraint stress (RST) 3 days before and for 5 days following dorsal application of a 3.5-mm sterile punch wound. Control mice were wounded, but not restrained. Using photography and image analysis, the rate of wound healing was compared between the two groups. Wounds on control mice healed on average 3.10 days sooner than RST-treated mice. In addition, cross-sectional, morphometric analysis of the dermal and epidermal layers revealed reduced inflammation surrounding wounds from RST mice at 1, 3, and 5 days after wounding. In the RST group, serum corticosterone levels averaged 162.5 ng/ml compared to 35.7 ng/ml in the controls. Treatment of RST-stressed animals with the glucocorticoid receptor antagonist RU40555 resulted in healing rates comparable to those of control animals. Thus, the reduction in inflammation and delayed healing correlated with serum corticosterone levels and suggest that disruption of neuroendocrine homeostasis modulates wound healing.

KW - Glucocorticoid

KW - Mice

KW - RU40555

KW - Stress

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=0012007921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0012007921&partnerID=8YFLogxK

U2 - 10.1006/brbi.1997.0512

DO - 10.1006/brbi.1997.0512

M3 - Article

VL - 12

SP - 64

EP - 73

JO - Brain, Behavior, and Immunity

JF - Brain, Behavior, and Immunity

SN - 0889-1591

IS - 1

ER -