Resting B lymphocytes as APC for naive T lymphocytes: Dependence on CD40 ligand/CD40

Dean E. Evans, Michael Munks, Jeffrey M. Purkerson, David Parker

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Although resting B cells as APC are tolerogenic for naive T cells in vivo, we show here that they can provide all the costimulatory signals necessary for naive T cell proliferation in vivo and in vitro. In the absence of an activating signal through the B cell Ag receptor, T cell proliferation after Ag recognition on resting B cells depends on CD40 expression on the B cells, implying that naive T cells use the membrane-bound cytokine, CD40 ligand (CD154), to induce the costimulatory signals that they need. Induction of B7-1 (CD80) and increased or sustained expression of CD44H, ICAM-1 (CD54), and B7-2 (CD86) are dependent on the interaction of CD40 ligand with CD40. Transient expression (12 h) of B7-2 is T cell- and peptide Ag-dependent, but CD40-independent. Only sustained (≥24 h) expression of B7-2 and perhaps increased expression of ICAM-1 could be shown to be functionally important in this system. T cells cultured with CD40-deficient B cells and peptide remain about as responsive as fresh naive cells upon secondary culture with whole splenic APC. Therefore, B cells, and perhaps other APC, may be tolerogenic not because they fail to provide sufficient costimulation for T cell proliferation, but because they are deficient in some later functions necessary for a productive T cell response.

Original languageEnglish (US)
Pages (from-to)688-697
Number of pages10
JournalJournal of Immunology
Volume164
Issue number2
StatePublished - Jan 15 2000

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CD40 Ligand
B-Lymphocytes
T-Lymphocytes
Cell Proliferation
Intercellular Adhesion Molecule-1
Peptides
Cell Membrane
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Resting B lymphocytes as APC for naive T lymphocytes : Dependence on CD40 ligand/CD40. / Evans, Dean E.; Munks, Michael; Purkerson, Jeffrey M.; Parker, David.

In: Journal of Immunology, Vol. 164, No. 2, 15.01.2000, p. 688-697.

Research output: Contribution to journalArticle

Evans, DE, Munks, M, Purkerson, JM & Parker, D 2000, 'Resting B lymphocytes as APC for naive T lymphocytes: Dependence on CD40 ligand/CD40', Journal of Immunology, vol. 164, no. 2, pp. 688-697.
Evans, Dean E. ; Munks, Michael ; Purkerson, Jeffrey M. ; Parker, David. / Resting B lymphocytes as APC for naive T lymphocytes : Dependence on CD40 ligand/CD40. In: Journal of Immunology. 2000 ; Vol. 164, No. 2. pp. 688-697.
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