Resting B lymphocytes as APC for naive T lymphocytes: Dependence on CD40 ligand/CD40

Dean E. Evans, Michael W. Munks, Jeffrey M. Purkerson, David C. Parker

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Although resting B cells as APC are tolerogenic for naive T cells in vivo, we show here that they can provide all the costimulatory signals necessary for naive T cell proliferation in vivo and in vitro. In the absence of an activating signal through the B cell Ag receptor, T cell proliferation after Ag recognition on resting B cells depends on CD40 expression on the B cells, implying that naive T cells use the membrane-bound cytokine, CD40 ligand (CD154), to induce the costimulatory signals that they need. Induction of B7-1 (CD80) and increased or sustained expression of CD44H, ICAM-1 (CD54), and B7-2 (CD86) are dependent on the interaction of CD40 ligand with CD40. Transient expression (12 h) of B7-2 is T cell- and peptide Ag-dependent, but CD40-independent. Only sustained (≥24 h) expression of B7-2 and perhaps increased expression of ICAM-1 could be shown to be functionally important in this system. T cells cultured with CD40-deficient B cells and peptide remain about as responsive as fresh naive cells upon secondary culture with whole splenic APC. Therefore, B cells, and perhaps other APC, may be tolerogenic not because they fail to provide sufficient costimulation for T cell proliferation, but because they are deficient in some later functions necessary for a productive T cell response.

Original languageEnglish (US)
Pages (from-to)688-697
Number of pages10
JournalJournal of Immunology
Volume164
Issue number2
DOIs
StatePublished - Jan 15 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Resting B lymphocytes as APC for naive T lymphocytes: Dependence on CD40 ligand/CD40'. Together they form a unique fingerprint.

  • Cite this