REST and its corepressors mediate plasticity of neuronal gene chromatin throughout neurogenesis

Nurit Ballas, Christopher Grunseich, Diane D. Lu, Joan C. Speh, Gail Mandel

Research output: Contribution to journalArticle

611 Scopus citations

Abstract

Regulation of neuronal gene expression is critical to central nervous system development. Here, we show that REST regulates the transitions from pluripotent to neural stem/progenitor cell and from progenitor to mature neuron. In the transition to progenitor cell, REST is degraded to levels just sufficient to maintain neuronal gene chromatin in an inactive state that is nonetheless poised for expression. As progenitors differentiate into neurons, REST and its corepressors dissociate from the RE1 site, triggering activation of neuronal genes. In some genes, the level of expression is adjusted further in neurons by CoREST/MeCP2 repressor complexes that remain bound to a site of methylated DNA distinct from the RE1 site. Expression profiling based on this mechanism indicates that REST defines a gene set subject to plasticity in mature neurons. Thus, a multistage repressor mechanism controls the orderly expression of genes during development while still permitting fine tuning in response to specific stimuli.

Original languageEnglish (US)
Pages (from-to)645-657
Number of pages13
JournalCell
Volume121
Issue number4
DOIs
StatePublished - May 20 2005

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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