Responses to the Human Intruder Test are related to hair cortisol phenotype and sex in rhesus macaques (Macaca mulatta)

Amanda F. Hamel, Corrine K. Lutz, Kristine Coleman, Julie M. Worlein, Emily J. Peterson, Kendra L. Rosenberg, Melinda A. Novak, Jerrold S. Meyer

    Research output: Contribution to journalArticle

    12 Scopus citations

    Abstract

    Measurement of cortisol in hair provides a chronic index of hypothalamic–pituitary–adrenal (HPA) axis activity and has been applied to assessments of temperament (stable behavioral differences between individuals). However, the extent to which chronically high HPA axis activity relates to a correspondingly high degree of behavioral reactivity is as yet unknown. Therefore, the goal of the present experiment was to assess the relationship between hair cortisol and a reactive temperament. We administered the Human Intruder Test (HIT) twice to 145 (80 male) rhesus macaques (Macaca mulatta) in order to assess behavioral reactivity. The HIT presents monkeys with an unfamiliar experimenter and is composed of a Baseline phase (no intruder) followed by three experimental phases in which the orientation of the intruder changes (Profile, Stare, Back). Behavioral responses to the test were videotaped and behaviors thought to reflect a reactive response to the intruder were scored for duration. Hair samples collected within ±1 month of the first HIT session were analyzed for cortisol by enzyme immunoassay. Subjects were assigned to three groups based on hair cortisol concentration: high, intermediate, and low cortisol phenotypes. Monkeys with the high cortisol phenotype were more reactive to the presence of the intruder than those with the low cortisol phenotype: they were more aggressive, scratched more, and spent more time in the back half of the cage. Males yawned significantly more while females spent more time immobile and in the back of the cage. Overall, monkeys with higher hair cortisol demonstrated an exaggerated response to the presence of the human intruder, supporting a relationship between high levels of chronic HPA axis activity and a reactive temperament. These results indicate that high levels of HPA axis activity, which may result from either genetic variation or environmental stress, correspond with heightened behavioral responses to a stressful experience. Am. J. Primatol. 79:e22526, 2017.

    Original languageEnglish (US)
    Pages (from-to)1-10
    Number of pages10
    JournalAmerican Journal of Primatology
    Volume79
    Issue number1
    DOIs
    StatePublished - Jan 1 2017

    Keywords

    • hair cortisol
    • human intruder test
    • hypothalamic–pituitary–adrenal axis
    • temperament

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Animal Science and Zoology

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    Hamel, A. F., Lutz, C. K., Coleman, K., Worlein, J. M., Peterson, E. J., Rosenberg, K. L., Novak, M. A., & Meyer, J. S. (2017). Responses to the Human Intruder Test are related to hair cortisol phenotype and sex in rhesus macaques (Macaca mulatta). American Journal of Primatology, 79(1), 1-10. https://doi.org/10.1002/ajp.22526