Response to 5-hydroxytryptamine on neurons of guinea pig celiac and inferior mesenteric ganglia in primary culture

Steven R. Knoper, Steven Matsumoto, David L. Kreulen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The electrophysiological effects of serotonin, a putative neurotransmitter in prevertebral sympathetic ganglia, were evaluated in cultured celiac and inferior mesenteric ganglia (IMG) neurons. Intracellular microelectrode recordings were performed in neurons that were maintained in culture an average of 26 days. Seventy-eight of 85 neurons responded when serotonin (10 μM) was applied by pressure ejection from a micropipette to the surface of the isolated cells. The majority of the neurons (n = 48) generated fast depolarizations, although slow depolarizations (n = 17), bipolar responses (n = 5), hyperpolarizations (n = 7), and a biphasic response (n = 1), were also seen. Hyperpolarizing responses were evoked in celiac neurons only. All responses were inhibited by the 5-HT3 antagonist MDL 72,222 (5 μM). Fast responses were not inhibited by tetrodotoxin (n = 3). These results demonstrate that serotonin evokes a variety of membrane potential changes in cultured prevertebral sympathetic neurons by activating 5-HT3 receptors.

Original languageEnglish (US)
Pages (from-to)703-710
Number of pages8
JournalLife Sciences
Volume51
Issue number9
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Ganglia
Abdomen
Neurons
Serotonin
Guinea Pigs
Depolarization
Serotonin 5-HT3 Receptor Antagonists
Receptors, Serotonin, 5-HT3
Sympathetic Ganglia
Microelectrodes
Tetrodotoxin
Membrane Potentials
Neurotransmitter Agents
Membranes
Pressure

ASJC Scopus subject areas

  • Pharmacology

Cite this

Response to 5-hydroxytryptamine on neurons of guinea pig celiac and inferior mesenteric ganglia in primary culture. / Knoper, Steven R.; Matsumoto, Steven; Kreulen, David L.

In: Life Sciences, Vol. 51, No. 9, 1992, p. 703-710.

Research output: Contribution to journalArticle

@article{4c992c53317b48b9bdbde115c95dd7ad,
title = "Response to 5-hydroxytryptamine on neurons of guinea pig celiac and inferior mesenteric ganglia in primary culture",
abstract = "The electrophysiological effects of serotonin, a putative neurotransmitter in prevertebral sympathetic ganglia, were evaluated in cultured celiac and inferior mesenteric ganglia (IMG) neurons. Intracellular microelectrode recordings were performed in neurons that were maintained in culture an average of 26 days. Seventy-eight of 85 neurons responded when serotonin (10 μM) was applied by pressure ejection from a micropipette to the surface of the isolated cells. The majority of the neurons (n = 48) generated fast depolarizations, although slow depolarizations (n = 17), bipolar responses (n = 5), hyperpolarizations (n = 7), and a biphasic response (n = 1), were also seen. Hyperpolarizing responses were evoked in celiac neurons only. All responses were inhibited by the 5-HT3 antagonist MDL 72,222 (5 μM). Fast responses were not inhibited by tetrodotoxin (n = 3). These results demonstrate that serotonin evokes a variety of membrane potential changes in cultured prevertebral sympathetic neurons by activating 5-HT3 receptors.",
author = "Knoper, {Steven R.} and Steven Matsumoto and Kreulen, {David L.}",
year = "1992",
doi = "10.1016/0024-3205(92)90244-J",
language = "English (US)",
volume = "51",
pages = "703--710",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Response to 5-hydroxytryptamine on neurons of guinea pig celiac and inferior mesenteric ganglia in primary culture

AU - Knoper, Steven R.

AU - Matsumoto, Steven

AU - Kreulen, David L.

PY - 1992

Y1 - 1992

N2 - The electrophysiological effects of serotonin, a putative neurotransmitter in prevertebral sympathetic ganglia, were evaluated in cultured celiac and inferior mesenteric ganglia (IMG) neurons. Intracellular microelectrode recordings were performed in neurons that were maintained in culture an average of 26 days. Seventy-eight of 85 neurons responded when serotonin (10 μM) was applied by pressure ejection from a micropipette to the surface of the isolated cells. The majority of the neurons (n = 48) generated fast depolarizations, although slow depolarizations (n = 17), bipolar responses (n = 5), hyperpolarizations (n = 7), and a biphasic response (n = 1), were also seen. Hyperpolarizing responses were evoked in celiac neurons only. All responses were inhibited by the 5-HT3 antagonist MDL 72,222 (5 μM). Fast responses were not inhibited by tetrodotoxin (n = 3). These results demonstrate that serotonin evokes a variety of membrane potential changes in cultured prevertebral sympathetic neurons by activating 5-HT3 receptors.

AB - The electrophysiological effects of serotonin, a putative neurotransmitter in prevertebral sympathetic ganglia, were evaluated in cultured celiac and inferior mesenteric ganglia (IMG) neurons. Intracellular microelectrode recordings were performed in neurons that were maintained in culture an average of 26 days. Seventy-eight of 85 neurons responded when serotonin (10 μM) was applied by pressure ejection from a micropipette to the surface of the isolated cells. The majority of the neurons (n = 48) generated fast depolarizations, although slow depolarizations (n = 17), bipolar responses (n = 5), hyperpolarizations (n = 7), and a biphasic response (n = 1), were also seen. Hyperpolarizing responses were evoked in celiac neurons only. All responses were inhibited by the 5-HT3 antagonist MDL 72,222 (5 μM). Fast responses were not inhibited by tetrodotoxin (n = 3). These results demonstrate that serotonin evokes a variety of membrane potential changes in cultured prevertebral sympathetic neurons by activating 5-HT3 receptors.

UR - http://www.scopus.com/inward/record.url?scp=0026696283&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026696283&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(92)90244-J

DO - 10.1016/0024-3205(92)90244-J

M3 - Article

VL - 51

SP - 703

EP - 710

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 9

ER -