Response of human T lymphocyte lines to myelin basic protein: Association of dominant epitopes with HLA class II restriction molecules

Y. K. Chou, M. Vainiene, R. Whitham, D. Bourdette, C. H.‐J Chou, G. Hashim, H. Offner, A. A. Vandenbark

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

In animals, the selection in vitro of T cell lines to myelin basic protein (MBP) can define immunodominant and encephalitogenic epitopes which are preferentially associated with class II major histocompatibility (MHC) molecules. These principles were used to evaluate the specificity and MHC restriction of 14 human MBP‐reactive T cell lines selected from normal individuals and patients with multiple sclerosis (MS) and other neurological diseases (OND). The four normal T cell lines recognized single, separate immunodominant MBP epitopes which were restricted by MHC molecules from the DR or in one case the DP class II locus. In contrast, the MS and OND T cell lines recognized multiple MBP epitopes, each in association with a discrete class II MHC molecule from the DR or DQ locus. Overall, HLA‐DR molecules were used preferentially to associate with epitopes on human MBP, restricting 26/33 responses. As predicted from animal studies, T cells from genetically disparate individuals responded to different immunodominant epitopes on human MBP in association with distinct MHC class II molecules. HLA‐DR2, which is overrepresented in MS patients, possessed an unusual capacity to restrict all eight epitopes identified on MBP in this study. These data provide the first evidence of genetically restricted human T cell recognition of potentially encephalitogenic epitopes of MBP.

Original languageEnglish (US)
Pages (from-to)207-216
Number of pages10
JournalJournal of Neuroscience Research
Volume23
Issue number2
DOIs
StatePublished - Jun 1989

Keywords

  • T cell lines
  • epitope specificity
  • human myelin basic protein
  • immunodominance

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Response of human T lymphocyte lines to myelin basic protein: Association of dominant epitopes with HLA class II restriction molecules'. Together they form a unique fingerprint.

  • Cite this