Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3

C. T. Bond, R. Sprengel, J. M. Bissonnette, W. A. Kaufmann, D. Pribnow, T. Neelands, T. Storck, M. Baetscher, J. Jerecic, J. Maylie, H. G. Knaus, P. H. Seeburg, J. P. Adelman

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Abstract

In excitable cells, small-conductance Ca2+-activated potassium channels (SK channels) are responsible for the slow after-hyperpolarization that often follows an action potential Three SK channel subunits have been molecularly characterized. The SK3 gene was targeted by homologous recombination for the insertion of a gene switch that permitted experimental regulation of SK3 expression while retaining normal SK3 promoter function. An absence of SK3 did not present overt phenotypic consequences. However, SK3 overexpression induced abnormal respiratory responses to hypoxia and compromised parturition. Both conditions were corrected by silencing the gene. The results implicate SK3 channels as potential therapeutic targets for disorders such as sleep apnea or sudden infant death syndrome and for regulating uterine contractions during labor.

Original languageEnglish (US)
Pages (from-to)1942-1946
Number of pages5
JournalScience
Volume289
Issue number5486
DOIs
StatePublished - Sep 15 2000

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Cite this

Bond, C. T., Sprengel, R., Bissonnette, J. M., Kaufmann, W. A., Pribnow, D., Neelands, T., Storck, T., Baetscher, M., Jerecic, J., Maylie, J., Knaus, H. G., Seeburg, P. H., & Adelman, J. P. (2000). Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3. Science, 289(5486), 1942-1946. https://doi.org/10.1126/science.289.5486.1942