Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3

C. T. Bond, R. Sprengel, J. M. Bissonnette, W. A. Kaufmann, D. Pribnow, T. Neelands, T. Storck, M. Baetscher, J. Jerecic, James Maylie, H. G. Knaus, P. H. Seeburg, John Adelman

Research output: Contribution to journalArticle

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Abstract

In excitable cells, small-conductance Ca2+-activated potassium channels (SK channels) are responsible for the slow after-hyperpolarization that often follows an action potential Three SK channel subunits have been molecularly characterized. The SK3 gene was targeted by homologous recombination for the insertion of a gene switch that permitted experimental regulation of SK3 expression while retaining normal SK3 promoter function. An absence of SK3 did not present overt phenotypic consequences. However, SK3 overexpression induced abnormal respiratory responses to hypoxia and compromised parturition. Both conditions were corrected by silencing the gene. The results implicate SK3 channels as potential therapeutic targets for disorders such as sleep apnea or sudden infant death syndrome and for regulating uterine contractions during labor.

Original languageEnglish (US)
Pages (from-to)1942-1946
Number of pages5
JournalScience
Volume289
Issue number5486
DOIs
StatePublished - Sep 15 2000

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Small-Conductance Calcium-Activated Potassium Channels
Switch Genes
Calcium-Activated Potassium Channels
Uterine Contraction
Sudden Infant Death
Insertional Mutagenesis
Homologous Recombination
Sleep Apnea Syndromes
Gene Silencing
Action Potentials
Respiration
Parturition
Genes
Therapeutics
Hypoxia

ASJC Scopus subject areas

  • General

Cite this

Bond, C. T., Sprengel, R., Bissonnette, J. M., Kaufmann, W. A., Pribnow, D., Neelands, T., ... Adelman, J. (2000). Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3. Science, 289(5486), 1942-1946. https://doi.org/10.1126/science.289.5486.1942

Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3. / Bond, C. T.; Sprengel, R.; Bissonnette, J. M.; Kaufmann, W. A.; Pribnow, D.; Neelands, T.; Storck, T.; Baetscher, M.; Jerecic, J.; Maylie, James; Knaus, H. G.; Seeburg, P. H.; Adelman, John.

In: Science, Vol. 289, No. 5486, 15.09.2000, p. 1942-1946.

Research output: Contribution to journalArticle

Bond, CT, Sprengel, R, Bissonnette, JM, Kaufmann, WA, Pribnow, D, Neelands, T, Storck, T, Baetscher, M, Jerecic, J, Maylie, J, Knaus, HG, Seeburg, PH & Adelman, J 2000, 'Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3', Science, vol. 289, no. 5486, pp. 1942-1946. https://doi.org/10.1126/science.289.5486.1942
Bond CT, Sprengel R, Bissonnette JM, Kaufmann WA, Pribnow D, Neelands T et al. Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3. Science. 2000 Sep 15;289(5486):1942-1946. https://doi.org/10.1126/science.289.5486.1942
Bond, C. T. ; Sprengel, R. ; Bissonnette, J. M. ; Kaufmann, W. A. ; Pribnow, D. ; Neelands, T. ; Storck, T. ; Baetscher, M. ; Jerecic, J. ; Maylie, James ; Knaus, H. G. ; Seeburg, P. H. ; Adelman, John. / Respiration and parturition affected by conditional overexpression of the Ca2+-activated K+ channel subunit, SK3. In: Science. 2000 ; Vol. 289, No. 5486. pp. 1942-1946.
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