TY - JOUR
T1 - Resident macrophages in the cochlear blood-labyrinth barrier and their renewal via migration of bone-marrow-derived cells
AU - Shi, Xiaorui
N1 - Funding Information:
This work was supported by the National Institute of Deafness and Other Communications Disorders (grants R01 DC00844, R03 DC008888-02, and P30 DC005983).
PY - 2010/10
Y1 - 2010/10
N2 - A large population of perivascular cells was found to be present in the area of the blood-labyrinth barrier in the stria vascularis of normal adult cochlea. The cells were identified as perivascular resident macrophages (PVMs), as they were positive for several macrophage surface molecules including F4/80, CD68, and CD11b. The macrophages, which were closely associated with microvessels and structurally intertwined with endothelial cells and pericytes, constitutively expressed scavenger receptor classes A1 and B 1 and accumulated blood-borne proteins such as horseradish peroxidase and acetylated low-density lipoprotein. The PVMs were demonstrated to proliferate slowly, as evidenced by the absence of 5-bromo-2-deoxyuridine (BrdU)-positive PVMs at 3-14 days in normal mice injected with BrdU. However, in irradiated mice, the majority of the PVMs turned over via bone-marrow-cell migration within a 10-month time-frame. The existence of PVMs in the vascular wall of the blood-labyrinth barrier might therefore serve as a source for progenitor cells for postnatal vasculogenesis and might contribute to the repair of damaged vessels in the context of a local inflammatory response.
AB - A large population of perivascular cells was found to be present in the area of the blood-labyrinth barrier in the stria vascularis of normal adult cochlea. The cells were identified as perivascular resident macrophages (PVMs), as they were positive for several macrophage surface molecules including F4/80, CD68, and CD11b. The macrophages, which were closely associated with microvessels and structurally intertwined with endothelial cells and pericytes, constitutively expressed scavenger receptor classes A1 and B 1 and accumulated blood-borne proteins such as horseradish peroxidase and acetylated low-density lipoprotein. The PVMs were demonstrated to proliferate slowly, as evidenced by the absence of 5-bromo-2-deoxyuridine (BrdU)-positive PVMs at 3-14 days in normal mice injected with BrdU. However, in irradiated mice, the majority of the PVMs turned over via bone-marrow-cell migration within a 10-month time-frame. The existence of PVMs in the vascular wall of the blood-labyrinth barrier might therefore serve as a source for progenitor cells for postnatal vasculogenesis and might contribute to the repair of damaged vessels in the context of a local inflammatory response.
KW - Cochlea
KW - Confocal laser microscopy
KW - Immunohistochemistry
KW - Mouse (C57BL/6J; C57Bl/6-Tg)
KW - Resident macrophage
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U2 - 10.1007/s00441-010-1040-2
DO - 10.1007/s00441-010-1040-2
M3 - Article
C2 - 20838812
AN - SCOPUS:77957692427
SN - 0302-766X
VL - 342
SP - 21
EP - 30
JO - Cell and tissue research
JF - Cell and tissue research
IS - 1
ER -