Research highlights: enhancing whole genome amplification using compartmentalization

Andy Tay, Rajan P. Kulkarni, Armin Karimi, Dino Di Carlo

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The ability to break up a larger liquid volume into an array of smaller confined volumes that do not chemically communicate is a key enabling technology driving microfluidic innovations. We highlight recent work using drop-based confinement to improve on whole genome amplification, reducing amplification bias and contaminant amplification by bringing reactions to saturation within each confined drop. We also highlight a complementary technique to target whole genome amplification to a subset of nucleic acids within a sample by combining drop-based PCR with sorting and downstream sequencing. These new approaches have the potential to enhance our ability to categorize the diversity of microorganisms (especially difficult to culture species) that contribute to complex microbial communities, and in particular assemble the individual genomes of the species involved in biologically and environmentally important microbiomes.

Original languageEnglish (US)
Pages (from-to)4379-4382
Number of pages4
JournalLab on a Chip
Volume15
Issue number23
DOIs
StatePublished - Oct 21 2015
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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