Requirement for Src family protein tyrosine kinases in G2 for fibroblast cell division

Serge Roche, Stefano Fumagalli, Sara Courtneidge

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

The protein tyrosine kinase c-Src is transiently activated at the transition from the G2 phase to mitosis in the cell cycle of mammalian fibroblasts. Fyn and Yes, the other members of the Src family present in fibroblasts, were also found to be activated at mitosis. In cells microinjected with a neutralizing antibody specific for Src, Fyn, and Yes (anti-cst.1) during G2, cell division was inhibited by 75 percent. The block occurred before nuclear envelope breakdown. Antibodies specific for phosphatidylinositol-3 kinase α and phospholipase C-γ1 had no effect. Microinjection of the Src homology 2 (SH2) domain of Fyn was also inhibitory. Functional redundancy between members of the Src family was observed; a Src-specific antibody had no effect in NIH 3T3 cells but inhibited cell division in fibroblasts in which the only functional Src family kinase was Src itself. Thus, Src family kinases and proteins associating with their SH2 domains are required for entry into mitosis.

Original languageEnglish (US)
Pages (from-to)1567-1569
Number of pages3
JournalScience
Volume269
Issue number5230
StatePublished - 1995
Externally publishedYes

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src-Family Kinases
Mitosis
Cell Division
src Homology Domains
Fibroblasts
Phosphatidylinositol 3-Kinase
NIH 3T3 Cells
Antibodies
G2 Phase
Nuclear Envelope
Microinjections
Type C Phospholipases
Neutralizing Antibodies
Cell Cycle
Proteins

ASJC Scopus subject areas

  • General

Cite this

Requirement for Src family protein tyrosine kinases in G2 for fibroblast cell division. / Roche, Serge; Fumagalli, Stefano; Courtneidge, Sara.

In: Science, Vol. 269, No. 5230, 1995, p. 1567-1569.

Research output: Contribution to journalArticle

Roche, Serge ; Fumagalli, Stefano ; Courtneidge, Sara. / Requirement for Src family protein tyrosine kinases in G2 for fibroblast cell division. In: Science. 1995 ; Vol. 269, No. 5230. pp. 1567-1569.
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