Reply: Whole-culture synchronization - Effective tools for cell cycle studies

Paul Spellman, Gavin Sherlock

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Studies of gene expression during the eukaryotic cell cycle in whole-culture synchronized cultures have been published using many methodologies. These procedures alter the state of the cell cycle for a population of cells, rather than purifying a population of cells that are in the same state. Criticism of these methods (e.g. see Cooper, this issue, pp. 266-269, DOI:10.1016/j.tibtech.2004.04.009) suggests that these studies are flawed, and posits that such methodologies cannot be used to study the cell cycle because they alter the size and age distributions of the cultures. We believe that whole-culture cell cycle studies work even though they alter the size and age distributions: these cells still progress through the cell cycle and although we do not suggest that the methods are perfect, we will explain how these microarray studies have successfully identified cell cycle regulated genes and why these results are biologically meaningful.

Original languageEnglish (US)
Pages (from-to)270-273
Number of pages4
JournalTrends in Biotechnology
Volume22
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Cell culture
Cell Cycle
Synchronization
Cells
Age Distribution
cdc Genes
Eukaryotic Cells
Population
Microarrays
Gene expression
Gene Expression
Genes

ASJC Scopus subject areas

  • Bioengineering

Cite this

Reply : Whole-culture synchronization - Effective tools for cell cycle studies. / Spellman, Paul; Sherlock, Gavin.

In: Trends in Biotechnology, Vol. 22, No. 6, 06.2004, p. 270-273.

Research output: Contribution to journalArticle

@article{a1bc9bb139064db1863d69a8ab84064b,
title = "Reply: Whole-culture synchronization - Effective tools for cell cycle studies",
abstract = "Studies of gene expression during the eukaryotic cell cycle in whole-culture synchronized cultures have been published using many methodologies. These procedures alter the state of the cell cycle for a population of cells, rather than purifying a population of cells that are in the same state. Criticism of these methods (e.g. see Cooper, this issue, pp. 266-269, DOI:10.1016/j.tibtech.2004.04.009) suggests that these studies are flawed, and posits that such methodologies cannot be used to study the cell cycle because they alter the size and age distributions of the cultures. We believe that whole-culture cell cycle studies work even though they alter the size and age distributions: these cells still progress through the cell cycle and although we do not suggest that the methods are perfect, we will explain how these microarray studies have successfully identified cell cycle regulated genes and why these results are biologically meaningful.",
author = "Paul Spellman and Gavin Sherlock",
year = "2004",
month = "6",
doi = "10.1016/j.tibtech.2004.04.010",
language = "English (US)",
volume = "22",
pages = "270--273",
journal = "Trends in Biotechnology",
issn = "0167-7799",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Reply

T2 - Whole-culture synchronization - Effective tools for cell cycle studies

AU - Spellman, Paul

AU - Sherlock, Gavin

PY - 2004/6

Y1 - 2004/6

N2 - Studies of gene expression during the eukaryotic cell cycle in whole-culture synchronized cultures have been published using many methodologies. These procedures alter the state of the cell cycle for a population of cells, rather than purifying a population of cells that are in the same state. Criticism of these methods (e.g. see Cooper, this issue, pp. 266-269, DOI:10.1016/j.tibtech.2004.04.009) suggests that these studies are flawed, and posits that such methodologies cannot be used to study the cell cycle because they alter the size and age distributions of the cultures. We believe that whole-culture cell cycle studies work even though they alter the size and age distributions: these cells still progress through the cell cycle and although we do not suggest that the methods are perfect, we will explain how these microarray studies have successfully identified cell cycle regulated genes and why these results are biologically meaningful.

AB - Studies of gene expression during the eukaryotic cell cycle in whole-culture synchronized cultures have been published using many methodologies. These procedures alter the state of the cell cycle for a population of cells, rather than purifying a population of cells that are in the same state. Criticism of these methods (e.g. see Cooper, this issue, pp. 266-269, DOI:10.1016/j.tibtech.2004.04.009) suggests that these studies are flawed, and posits that such methodologies cannot be used to study the cell cycle because they alter the size and age distributions of the cultures. We believe that whole-culture cell cycle studies work even though they alter the size and age distributions: these cells still progress through the cell cycle and although we do not suggest that the methods are perfect, we will explain how these microarray studies have successfully identified cell cycle regulated genes and why these results are biologically meaningful.

UR - http://www.scopus.com/inward/record.url?scp=2442479598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442479598&partnerID=8YFLogxK

U2 - 10.1016/j.tibtech.2004.04.010

DO - 10.1016/j.tibtech.2004.04.010

M3 - Article

C2 - 15158053

AN - SCOPUS:2442479598

VL - 22

SP - 270

EP - 273

JO - Trends in Biotechnology

JF - Trends in Biotechnology

SN - 0167-7799

IS - 6

ER -