Repeated electroconvulsive shock or chronic morphine treatment increases the number of 3HDALA2, DLEU5enkephalin binding sites in rat brain membranes

John W. Holaday, Robert Hitzemann, Jim Curell, Frank C. Tortella, Gregory Lucas Belenky

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Experiments were performed in rats to evaluate the possible mechanisms responsible for the pharmacological cross-sensitization observed between repeated electroconvulsive shock (ECS) and chronic morphine administration. Repeated daily ECS for 9 days as well as chronic morphine pellet implantation resulted in a significant increase in the number of 3H-DADLE binding sites (Bmax values of 231 and 196 fmoles/mg protein, respectively). By contrast, single ECS, repeated sham ECS, and placebo pellet-treated rats all had significantly lower Bmax values (approx. 170 fmoles/mg protein). Affinities were not significantly altered by these treatments (Kd values between 3.1 and 4.0 nM). These data may link pharmacological cross-sensitization (repeated ECS and chronic morphine treatment) with a functional increase in the number of available opioid receptors.

Original languageEnglish (US)
Pages (from-to)2359-2362
Number of pages4
JournalLife Sciences
Volume31
Issue number20-21
DOIs
StatePublished - 1982
Externally publishedYes

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Electroshock
Morphine
Rats
Brain
Binding Sites
Membranes
Leucine-2-Alanine Enkephalin
Opioid Receptors
Pharmacology
Proteins
Therapeutics
Placebos
Experiments

ASJC Scopus subject areas

  • Pharmacology

Cite this

Repeated electroconvulsive shock or chronic morphine treatment increases the number of 3HDALA2, DLEU5enkephalin binding sites in rat brain membranes. / Holaday, John W.; Hitzemann, Robert; Curell, Jim; Tortella, Frank C.; Belenky, Gregory Lucas.

In: Life Sciences, Vol. 31, No. 20-21, 1982, p. 2359-2362.

Research output: Contribution to journalArticle

Holaday, John W. ; Hitzemann, Robert ; Curell, Jim ; Tortella, Frank C. ; Belenky, Gregory Lucas. / Repeated electroconvulsive shock or chronic morphine treatment increases the number of 3HDALA2, DLEU5enkephalin binding sites in rat brain membranes. In: Life Sciences. 1982 ; Vol. 31, No. 20-21. pp. 2359-2362.
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