Repeated electroconvulsive shock or chronic morphine treatment increases the number of 3HDALA2, DLEU5enkephalin binding sites in rat brain membranes

John W. Holaday, Robert J. Hitzemann, Jim Curell, Frank C. Tortella, Gregory Lucas Belenky

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Experiments were performed in rats to evaluate the possible mechanisms responsible for the pharmacological cross-sensitization observed between repeated electroconvulsive shock (ECS) and chronic morphine administration. Repeated daily ECS for 9 days as well as chronic morphine pellet implantation resulted in a significant increase in the number of 3H-DADLE binding sites (Bmax values of 231 and 196 fmoles/mg protein, respectively). By contrast, single ECS, repeated sham ECS, and placebo pellet-treated rats all had significantly lower Bmax values (approx. 170 fmoles/mg protein). Affinities were not significantly altered by these treatments (Kd values between 3.1 and 4.0 nM). These data may link pharmacological cross-sensitization (repeated ECS and chronic morphine treatment) with a functional increase in the number of available opioid receptors.

Original languageEnglish (US)
Pages (from-to)2359-2362
Number of pages4
JournalLife Sciences
Volume31
Issue number20-21
DOIs
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Fingerprint Dive into the research topics of 'Repeated electroconvulsive shock or chronic morphine treatment increases the number of <sup>3</sup>HDALA<sup>2</sup>, DLEU<sup>5</sup>enkephalin binding sites in rat brain membranes'. Together they form a unique fingerprint.

  • Cite this