Repair of psoralen interstrand cross-links in Xenopus laevis egg extracts is highly mutagenic

Xiaoyan Lu, Nianxiang Zhang, Karen Vasquez, Michelle Barton, Randy Legerski

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The recognition and removal of interstrand cross-links is perhaps the least understood of all repair pathways in eukaryotic cells. We have shown previously that uncoupling of cross-links occurs in mammalian cell extracts and have identified a number of factors that mediate this process. However, we have not observed complete repair of the substrate in this system. Here, we show that uncoupling of interstrand cross-links also occurs in Xenopus laevis egg extracts, and that the initial products of this reaction are identical to the products observed in mammalian cell extracts suggesting a common mechanism. However in contrast to mammalian cell extracts, we observe repair of the cross-linked substrate in the Xenopus extracts presumably by a translesion bypass mechanism that allows replication past the uncoupled monoadduct, and its likely subsequent removal by nucleotide excision repair. This repair process is shown to be highly mutagenic consistent with bypass synthesis.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume336
Issue number1
DOIs
StatePublished - Oct 14 2005
Externally publishedYes

Keywords

  • DNA repair
  • Interstrand cross-links
  • Mutagenesis
  • Xenopus laevis extract

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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