Repair of bleomycin-damaged DNA by human fibroblasts

M. M. Hurt, A. L. Beaudet, Robb Moses

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

The ability of human fibroblasts to repair bleomycin damaged DNA was examined in vivo. Repair of the specific lesions caused by bleomycin (BLM) was investigated in normal cell strains as well as those isolated from patients with apparent DNA repair defects. The diseases ataxia telangiectasia (AT), Bloom syndrome (BS), Cockayne syndrome (CS), Fanconi anemia (FA), and xeroderma pigmentosum (XP) were those selected for study. The method used for studying the repair of DNA after BLM exposure was alkaline sucrose gradient centrifugation. After exposure to BLM, a fall in the molecular weight of DNA was observed, and after removal the DNA reformed rapidly to high molecular weight. The fall in molecular weight upon exposure to BLM ws observed in all cells examined with the exception of some XP strains. Prelabeled cells from some XP complementation groups were found to have a higher percentage of low molecular weight DNA on alkaline gradients than did normal cells. This prelabeled low molecular weight DNA disappeared upon exposure to BLM.

Original languageEnglish (US)
Title of host publicationJournal of Supramolecular and Cellular Biochemistry
Pages303-309
Number of pages7
Volume16
Edition4
StatePublished - 1981
Externally publishedYes

Fingerprint

Bleomycin
Fibroblasts
Repair
Xeroderma Pigmentosum
Molecular Weight
DNA
Molecular weight
DNA Repair
Cockayne Syndrome
Bloom Syndrome
Fanconi Anemia
Ataxia Telangiectasia
Centrifugation
Sucrose
Defects

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hurt, M. M., Beaudet, A. L., & Moses, R. (1981). Repair of bleomycin-damaged DNA by human fibroblasts. In Journal of Supramolecular and Cellular Biochemistry (4 ed., Vol. 16, pp. 303-309)

Repair of bleomycin-damaged DNA by human fibroblasts. / Hurt, M. M.; Beaudet, A. L.; Moses, Robb.

Journal of Supramolecular and Cellular Biochemistry. Vol. 16 4. ed. 1981. p. 303-309.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hurt, MM, Beaudet, AL & Moses, R 1981, Repair of bleomycin-damaged DNA by human fibroblasts. in Journal of Supramolecular and Cellular Biochemistry. 4 edn, vol. 16, pp. 303-309.
Hurt MM, Beaudet AL, Moses R. Repair of bleomycin-damaged DNA by human fibroblasts. In Journal of Supramolecular and Cellular Biochemistry. 4 ed. Vol. 16. 1981. p. 303-309
Hurt, M. M. ; Beaudet, A. L. ; Moses, Robb. / Repair of bleomycin-damaged DNA by human fibroblasts. Journal of Supramolecular and Cellular Biochemistry. Vol. 16 4. ed. 1981. pp. 303-309
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