TY - JOUR
T1 - Repair of bleomycin-damaged DNA by human fibroblasts
AU - Hurt, M. M.
AU - Beaudet, A. L.
AU - Moses, R. E.
PY - 1981
Y1 - 1981
N2 - The ability of human fibroblasts to repair bleomycin damaged DNA was examined in vivo. Repair of the specific lesions caused by bleomycin (BLM) was investigated in normal cell strains as well as those isolated from patients with apparent DNA repair defects. The diseases ataxia telangiectasia (AT), Bloom syndrome (BS), Cockayne syndrome (CS), Fanconi anemia (FA), and xeroderma pigmentosum (XP) were those selected for study. The method used for studying the repair of DNA after BLM exposure was alkaline sucrose gradient centrifugation. After exposure to BLM, a fall in the molecular weight of DNA was observed, and after removal the DNA reformed rapidly to high molecular weight. The fall in molecular weight upon exposure to BLM ws observed in all cells examined with the exception of some XP strains. Prelabeled cells from some XP complementation groups were found to have a higher percentage of low molecular weight DNA on alkaline gradients than did normal cells. This prelabeled low molecular weight DNA disappeared upon exposure to BLM.
AB - The ability of human fibroblasts to repair bleomycin damaged DNA was examined in vivo. Repair of the specific lesions caused by bleomycin (BLM) was investigated in normal cell strains as well as those isolated from patients with apparent DNA repair defects. The diseases ataxia telangiectasia (AT), Bloom syndrome (BS), Cockayne syndrome (CS), Fanconi anemia (FA), and xeroderma pigmentosum (XP) were those selected for study. The method used for studying the repair of DNA after BLM exposure was alkaline sucrose gradient centrifugation. After exposure to BLM, a fall in the molecular weight of DNA was observed, and after removal the DNA reformed rapidly to high molecular weight. The fall in molecular weight upon exposure to BLM ws observed in all cells examined with the exception of some XP strains. Prelabeled cells from some XP complementation groups were found to have a higher percentage of low molecular weight DNA on alkaline gradients than did normal cells. This prelabeled low molecular weight DNA disappeared upon exposure to BLM.
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U2 - 10.1002/jsscb.1981.380160402
DO - 10.1002/jsscb.1981.380160402
M3 - Article
C2 - 6171650
AN - SCOPUS:0019867449
SN - 0730-2312
VL - 16
SP - 303
EP - 309
JO - Journal of Supramolecular and Cellular Biochemistry
JF - Journal of Supramolecular and Cellular Biochemistry
IS - 4
ER -