Renal retention of lipid microbubbles: A potential mechanism for flank discomfort during ultrasound contrast administration

Ya Ni Liu, Jaspreet Khangura, Aris Xie, J. Todd Belcik, Qi Yue, Brian P. Davidson, Yan Zhao, Sajeevani Kim, Yoichi Inaba, Jonathan Lindner

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Background The etiology of flank pain sometimes experienced during the administration of ultrasound contrast agents is unknown. The aim of this study was to investigate whether microbubble ultrasound contrast agents are retained within the renal microcirculation, which could lead to either flow disturbance or local release of vasoactive and pain mediators downstream from complement activation. Methods Retention of lipid-shelled microbubbles in the renal microcirculation of mice was assessed by confocal fluorescent microscopy and contrast-enhanced ultrasound imaging with dose-escalating intravenous injection. Studies were performed with size-segregated microbubbles to investigate physical entrapment, after glycocalyx degradation and in wild-type and C3-deficient mice to investigate complement-mediated retention. Urinary bradykinin was measured before and after microbubble administrations. Renal contrast-enhanced ultrasound in human subjects (n = 13) was performed 7 to 10 min after the completion of lipid microbubble administration. Results In both mice and humans, microbubble retention was detected in the renal cortex by persistent contrast-enhanced ultrasound signal enhancement. Microbubble retention in mice was linearly related to dose and occurred almost exclusively in cortical glomerular microvessels. Microbubble retention did not affect microsphere-derived renal blood flow. Microbubble retention was not influenced by glycocalyx degradation or by microbubble size, thereby excluding lodging, but was reduced by 90% (P

    Original languageEnglish (US)
    Pages (from-to)1474-1481
    Number of pages8
    JournalJournal of the American Society of Echocardiography
    Volume26
    Issue number12
    DOIs
    StatePublished - Dec 2013

    Fingerprint

    Microbubbles
    Kidney
    Lipids
    Glycocalyx
    Microcirculation
    Contrast Media
    Flank Pain
    Complement Activation
    Renal Circulation
    Bradykinin
    Microvessels
    Microspheres
    Confocal Microscopy
    Intravenous Injections
    Ultrasonography
    Pain

    Keywords

    • Complement
    • Contrast echocardiography
    • Microbubbles
    • Safety

    ASJC Scopus subject areas

    • Radiology Nuclear Medicine and imaging
    • Cardiology and Cardiovascular Medicine

    Cite this

    Renal retention of lipid microbubbles : A potential mechanism for flank discomfort during ultrasound contrast administration. / Liu, Ya Ni; Khangura, Jaspreet; Xie, Aris; Belcik, J. Todd; Yue, Qi; Davidson, Brian P.; Zhao, Yan; Kim, Sajeevani; Inaba, Yoichi; Lindner, Jonathan.

    In: Journal of the American Society of Echocardiography, Vol. 26, No. 12, 12.2013, p. 1474-1481.

    Research output: Contribution to journalArticle

    Liu, Ya Ni ; Khangura, Jaspreet ; Xie, Aris ; Belcik, J. Todd ; Yue, Qi ; Davidson, Brian P. ; Zhao, Yan ; Kim, Sajeevani ; Inaba, Yoichi ; Lindner, Jonathan. / Renal retention of lipid microbubbles : A potential mechanism for flank discomfort during ultrasound contrast administration. In: Journal of the American Society of Echocardiography. 2013 ; Vol. 26, No. 12. pp. 1474-1481.
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    AU - Belcik, J. Todd

    AU - Yue, Qi

    AU - Davidson, Brian P.

    AU - Zhao, Yan

    AU - Kim, Sajeevani

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