Renal proximal tubular cells acquire resistance to cell death stimuli in mice with hereditary tyrosinemia type 1

Marjanka C. Luijerink, Ellen A.C.M. Van Beurden, Helga E.M. Malingré, Saskia M.M. Jacobs, Markus Grompe, Leo W.J. Klomp, Ruud Berger, Inge E.T. Van Den Berg

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background. Hereditary tyrosinemia type 1 (HT1), which is associated with severe liver and kidney damage, is caused by deficiency of fumarylacetoacetate hydrolase (FAH), the last enzyme of the tyrosine breakdown cascade. HT1-associated liver and kidney failure can be prevented by blocking an enzyme upstream of FAH in the tyrosine breakdown pathway with 2-(2-nitro-4- trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC). FAH knockout mice develop the HT1 phenotype when NTBC treatment is discontinued. Methods. The occurrence of cell death was investigated in kidneys of Fah-l- mice on and off NTBC either unchallenged or injected with 800 mg/kg of homogentisic acid (HGA), an intermediate of tyrosine breakdown. Results. No cell death could be detected in kidneys of Fah-l- mice on NTBC. A slight increase of cleaved caspase-3 was the only apoptosis-related feature that could be detected in kidneys of Fah-/- mice off NTBC. Challenge of Fah-/- mice on NTBC with HGA led to massive death of renal proximal tubular cells, with positive terminal deoxynucleotidyl transferase-mediated deoxyuridine diphosphate (dUDP) nick-end labeling (TUNEL) and DNA fragmentation assays, but hardly any cleavage of caspase-9 and caspase-3. Fah-l- mice off NTBC acquired resistance to HGA-induced renal cell death and the kidneys exhibited relatively few features of apoptosis upon challenge with HGA, with a small increase in expression of cleaved caspase-9 and caspase-3. Conclusion. Kidneys of adult Fah-/- mice, withdrawn from NTBC for 15 days, reveal limited characteristics of apoptosis, and have acquired resistance to a caspase-9- and caspase-3-independent form of cell death provoked by HGA.

Original languageEnglish (US)
Pages (from-to)990-1000
Number of pages11
JournalKidney International
Volume66
Issue number3
DOIs
StatePublished - Sep 2004

Keywords

  • Acquired resistance to cell death
  • Apoptotic cell death
  • Caspase-3
  • Caspase-9
  • Hereditary tyrosinemia type 1
  • Kidney damage

ASJC Scopus subject areas

  • Nephrology

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    Luijerink, M. C., Van Beurden, E. A. C. M., Malingré, H. E. M., Jacobs, S. M. M., Grompe, M., Klomp, L. W. J., Berger, R., & Van Den Berg, I. E. T. (2004). Renal proximal tubular cells acquire resistance to cell death stimuli in mice with hereditary tyrosinemia type 1. Kidney International, 66(3), 990-1000. https://doi.org/10.1111/j.1523-1755.2004.00788.x