Renal mitochondrial integrity during continuous gentamicin treatment

Leena M. Mela-Riker, Linda L. Widener, Donald Houghton, William M. Bennett

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Rats were given gentamicin over a period of 21 days. At 5, 10,14 and 21 days renal cortical mitochondria were isolated, and respiratory and Ca2+ transport functions and cytochrome concentrations were determined. The mitochondrial data were correlated with indicators of deteriorating renal function and tissue gentamicin accumulation. During the first 10 days of chronic gentamicin treatment, mitochondrial cytochrome oxidase and cytochrome c concentrations declined significantly. This decline was followed by a partial spontaneous recovery by days 14 and 21. Cytochrome b concentration was not significantly different from normal. Parallel with the cytochrome concentration changes, State 3 respiratory activities with all substrates studied and the rates of Ca2+ accumulation declined during the first 10 days and recovered spontaneously thereafter. It is concluded that chronic gentamicin treatment leading to renal failure inhibits mitochondrial energy-linked functions, which inhibition is induced by rate-limiting synthesis of those mitochondrial respiratory chain enzymes coded outside the mitochondrion.

Original languageEnglish (US)
Pages (from-to)979-984
Number of pages6
JournalBiochemical Pharmacology
Volume35
Issue number6
DOIs
StatePublished - Mar 15 1986

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Gentamicins
Kidney
Mitochondria
Cytochromes
Respiratory Transport
Cytochromes b
Electron Transport Complex IV
Electron Transport
Cytochromes c
Renal Insufficiency
Rats
Tissue
Recovery
Substrates
Enzymes

ASJC Scopus subject areas

  • Pharmacology

Cite this

Renal mitochondrial integrity during continuous gentamicin treatment. / Mela-Riker, Leena M.; Widener, Linda L.; Houghton, Donald; Bennett, William M.

In: Biochemical Pharmacology, Vol. 35, No. 6, 15.03.1986, p. 979-984.

Research output: Contribution to journalArticle

Mela-Riker, Leena M. ; Widener, Linda L. ; Houghton, Donald ; Bennett, William M. / Renal mitochondrial integrity during continuous gentamicin treatment. In: Biochemical Pharmacology. 1986 ; Vol. 35, No. 6. pp. 979-984.
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