TY - JOUR
T1 - Renal mitochondrial integrity during continuous gentamicin treatment
AU - Mela-Riker, Leena M.
AU - Widener, Linda L.
AU - Houghton, Donald C.
AU - Bennett, William M.
N1 - Funding Information:
Acknowledgements--The authortsh ankJ osephP . Pulliam, M.D. andJ an English,B .S., for technicaals sistancDe;a vid N. Gilbertf or performancoef gentamicians saysa;n dJ oni Utterbackfo r secretariahle lp.This work wass upported, in part,b yN ephrologRye searcAhs sociatesP,o rtlandO, R, and a grantf rom the NationalI nstituteso f Health,G M 33267.
PY - 1986/3/15
Y1 - 1986/3/15
N2 - Rats were given gentamicin over a period of 21 days. At 5, 10,14 and 21 days renal cortical mitochondria were isolated, and respiratory and Ca2+ transport functions and cytochrome concentrations were determined. The mitochondrial data were correlated with indicators of deteriorating renal function and tissue gentamicin accumulation. During the first 10 days of chronic gentamicin treatment, mitochondrial cytochrome oxidase and cytochrome c concentrations declined significantly. This decline was followed by a partial spontaneous recovery by days 14 and 21. Cytochrome b concentration was not significantly different from normal. Parallel with the cytochrome concentration changes, State 3 respiratory activities with all substrates studied and the rates of Ca2+ accumulation declined during the first 10 days and recovered spontaneously thereafter. It is concluded that chronic gentamicin treatment leading to renal failure inhibits mitochondrial energy-linked functions, which inhibition is induced by rate-limiting synthesis of those mitochondrial respiratory chain enzymes coded outside the mitochondrion.
AB - Rats were given gentamicin over a period of 21 days. At 5, 10,14 and 21 days renal cortical mitochondria were isolated, and respiratory and Ca2+ transport functions and cytochrome concentrations were determined. The mitochondrial data were correlated with indicators of deteriorating renal function and tissue gentamicin accumulation. During the first 10 days of chronic gentamicin treatment, mitochondrial cytochrome oxidase and cytochrome c concentrations declined significantly. This decline was followed by a partial spontaneous recovery by days 14 and 21. Cytochrome b concentration was not significantly different from normal. Parallel with the cytochrome concentration changes, State 3 respiratory activities with all substrates studied and the rates of Ca2+ accumulation declined during the first 10 days and recovered spontaneously thereafter. It is concluded that chronic gentamicin treatment leading to renal failure inhibits mitochondrial energy-linked functions, which inhibition is induced by rate-limiting synthesis of those mitochondrial respiratory chain enzymes coded outside the mitochondrion.
UR - http://www.scopus.com/inward/record.url?scp=0022480029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022480029&partnerID=8YFLogxK
U2 - 10.1016/0006-2952(86)90086-9
DO - 10.1016/0006-2952(86)90086-9
M3 - Article
C2 - 3006697
AN - SCOPUS:0022480029
SN - 0006-2952
VL - 35
SP - 979
EP - 984
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 6
ER -