Remodeling of the mammary microenvironment after lactation promotes breast tumor cell metastasis

Shauntae M. McDaniel, Kristen K. Rumer, Sandra L. Biroc, Richard P. Metz, Meenakshi Singh, Weston Porter, Pepper Schedin

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

The mammary gland microenvironment during post-lactational involution shares similarities with inflammation, including high matrix metalloproteinase activity, fibrillar collagen deposition, and release of bioactive fragments of fibronectin and laminin. Because inflammation can promote tumorigenesis, we evaluated whether the tissue microenvironment of the involuting gland is also promotional. Extracellular matrix was isolated from mammary glands of nulliparous rats or rats with mammary glands undergoing weaning-induced involution. Using these matrices as substratum, nulliparous matrix was found to promote ductal organization of normal mammary epithelial MCF-12A cells in three-dimensional culture and to suppress invasion of mammary tumor MDA-MB-231 cells in transwell filter assays. Conversely, involution matrix failed to support ductal development in normal cells and promoted invasiveness in tumor cells. To evaluate the effects of these matrices on metastasis in vivo, MDA-MB-231 cells, premixed with Matrigel, nulliparous matrix, or involution matrix, were injected into mammary fat pads of nude mice. Metastases to lung, liver, and kidney were increased in the involution matrix group, and correlated with a two-fold increase in tumor vascular endothelial growth factor expression and increased angiogenesis. These data suggest that the mammary gland microenvironment becomes promotional for tumor cell dissemination during involution, thus providing a plausible mechanism to explain the high rate of metastases that occur with pregnancy-associated breast cancer.

Original languageEnglish (US)
Pages (from-to)608-620
Number of pages13
JournalAmerican Journal of Pathology
Volume168
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

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Lactation
Breast
Human Mammary Glands
Breast Neoplasms
Neoplasm Metastasis
Fibrillar Collagens
Inflammation
Neoplasms
Laminin
Weaning
Matrix Metalloproteinases
Fibronectins
Nude Mice
Vascular Endothelial Growth Factor A
Extracellular Matrix
Adipose Tissue
Carcinogenesis
Kidney
Pregnancy
Lung

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Remodeling of the mammary microenvironment after lactation promotes breast tumor cell metastasis. / McDaniel, Shauntae M.; Rumer, Kristen K.; Biroc, Sandra L.; Metz, Richard P.; Singh, Meenakshi; Porter, Weston; Schedin, Pepper.

In: American Journal of Pathology, Vol. 168, No. 2, 02.2006, p. 608-620.

Research output: Contribution to journalArticle

McDaniel, Shauntae M. ; Rumer, Kristen K. ; Biroc, Sandra L. ; Metz, Richard P. ; Singh, Meenakshi ; Porter, Weston ; Schedin, Pepper. / Remodeling of the mammary microenvironment after lactation promotes breast tumor cell metastasis. In: American Journal of Pathology. 2006 ; Vol. 168, No. 2. pp. 608-620.
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