Abstract
In small presynaptic boutons in brain, synaptic vesicles are thought not to merge with the plasma membrane when they release transmitter, but instead to close their fusion pores and survive intact for future use (kiss-and-run exocytosis). The strongest evidence for this idea is the slow and incomplete release of the fluorescent membrane marker, FM1-43 [N-(3-triethylammoniumpropyl) -4-(4-(dibutylamino)styryl) pyridinium dibromide], from single vesicles. We investigated the release of FM1-43 from sparse cultures of hippocampal neurons grown on coverslips with no glia. This allowed presynaptic boutons to be imaged at favorable signal-to-noise ratio. Sparingly stained boutons were imaged at high time resolution, while high-frequency electrical stimulation caused exocytosis. The release of FM1-43 was quantal and occurred in abrupt steps, each representing a single fusion event. The fluorescence of vesicle clusters traveling along axons had a distribution with the same quantal size, indicating that a vesicle releases all the dye it contains. In most fusion events, the time constant of dye release was
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1894-1903 |
| Number of pages | 10 |
| Journal | Journal of Neuroscience |
| Volume | 28 |
| Issue number | 8 |
| DOIs | |
| State | Published - Feb 20 2008 |
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Keywords
- Fluorescence microscopy
- Hippocampus
- Kiss-and-run
- Presynaptic terminal
- Quantal release
- Vesicle transport
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Release of the styryl dyes from single synaptic vesicles in hippocampal neurons. / Chen, Xi; Barg, Sebastian; Almers, Wolfhard.
In: Journal of Neuroscience, Vol. 28, No. 8, 20.02.2008, p. 1894-1903.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Release of the styryl dyes from single synaptic vesicles in hippocampal neurons
AU - Chen, Xi
AU - Barg, Sebastian
AU - Almers, Wolfhard
PY - 2008/2/20
Y1 - 2008/2/20
N2 - In small presynaptic boutons in brain, synaptic vesicles are thought not to merge with the plasma membrane when they release transmitter, but instead to close their fusion pores and survive intact for future use (kiss-and-run exocytosis). The strongest evidence for this idea is the slow and incomplete release of the fluorescent membrane marker, FM1-43 [N-(3-triethylammoniumpropyl) -4-(4-(dibutylamino)styryl) pyridinium dibromide], from single vesicles. We investigated the release of FM1-43 from sparse cultures of hippocampal neurons grown on coverslips with no glia. This allowed presynaptic boutons to be imaged at favorable signal-to-noise ratio. Sparingly stained boutons were imaged at high time resolution, while high-frequency electrical stimulation caused exocytosis. The release of FM1-43 was quantal and occurred in abrupt steps, each representing a single fusion event. The fluorescence of vesicle clusters traveling along axons had a distribution with the same quantal size, indicating that a vesicle releases all the dye it contains. In most fusion events, the time constant of dye release was
AB - In small presynaptic boutons in brain, synaptic vesicles are thought not to merge with the plasma membrane when they release transmitter, but instead to close their fusion pores and survive intact for future use (kiss-and-run exocytosis). The strongest evidence for this idea is the slow and incomplete release of the fluorescent membrane marker, FM1-43 [N-(3-triethylammoniumpropyl) -4-(4-(dibutylamino)styryl) pyridinium dibromide], from single vesicles. We investigated the release of FM1-43 from sparse cultures of hippocampal neurons grown on coverslips with no glia. This allowed presynaptic boutons to be imaged at favorable signal-to-noise ratio. Sparingly stained boutons were imaged at high time resolution, while high-frequency electrical stimulation caused exocytosis. The release of FM1-43 was quantal and occurred in abrupt steps, each representing a single fusion event. The fluorescence of vesicle clusters traveling along axons had a distribution with the same quantal size, indicating that a vesicle releases all the dye it contains. In most fusion events, the time constant of dye release was
KW - Fluorescence microscopy
KW - Hippocampus
KW - Kiss-and-run
KW - Presynaptic terminal
KW - Quantal release
KW - Vesicle transport
UR - http://www.scopus.com/inward/record.url?scp=39749087484&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39749087484&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.4518-07.2008
DO - 10.1523/JNEUROSCI.4518-07.2008
M3 - Article
VL - 28
SP - 1894
EP - 1903
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 8
ER -