Release of the styryl dyes from single synaptic vesicles in hippocampal neurons

Xi Chen, Sebastian Barg, Wolfhard Almers

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

In small presynaptic boutons in brain, synaptic vesicles are thought not to merge with the plasma membrane when they release transmitter, but instead to close their fusion pores and survive intact for future use (kiss-and-run exocytosis). The strongest evidence for this idea is the slow and incomplete release of the fluorescent membrane marker, FM1-43 [N-(3-triethylammoniumpropyl) -4-(4-(dibutylamino)styryl) pyridinium dibromide], from single vesicles. We investigated the release of FM1-43 from sparse cultures of hippocampal neurons grown on coverslips with no glia. This allowed presynaptic boutons to be imaged at favorable signal-to-noise ratio. Sparingly stained boutons were imaged at high time resolution, while high-frequency electrical stimulation caused exocytosis. The release of FM1-43 was quantal and occurred in abrupt steps, each representing a single fusion event. The fluorescence of vesicle clusters traveling along axons had a distribution with the same quantal size, indicating that a vesicle releases all the dye it contains. In most fusion events, the time constant of dye release was

Original languageEnglish (US)
Pages (from-to)1894-1903
Number of pages10
JournalJournal of Neuroscience
Volume28
Issue number8
DOIs
StatePublished - Feb 20 2008

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Synaptic Vesicles
Coloring Agents
Exocytosis
Neurons
Signal-To-Noise Ratio
Neuroglia
Electric Stimulation
Axons
Fluorescence
Cell Membrane
Membranes
Brain
FM1 43

Keywords

  • Fluorescence microscopy
  • Hippocampus
  • Kiss-and-run
  • Presynaptic terminal
  • Quantal release
  • Vesicle transport

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Release of the styryl dyes from single synaptic vesicles in hippocampal neurons. / Chen, Xi; Barg, Sebastian; Almers, Wolfhard.

In: Journal of Neuroscience, Vol. 28, No. 8, 20.02.2008, p. 1894-1903.

Research output: Contribution to journalArticle

Chen, Xi ; Barg, Sebastian ; Almers, Wolfhard. / Release of the styryl dyes from single synaptic vesicles in hippocampal neurons. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 8. pp. 1894-1903.
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