RELEASE OF [3H]GABA FROM RAT CORTICAL SLICES: NEURONAL VS GLIAL ORIGIN

J. P. Hammerstad, C. R. Lyte

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Abstract— The effect of L‐2,4 diaminobutyric acid (DABA) and β‐alanine on the K+ stimulated release of [3H]GABA was examined using a continuous superfusion system in which a carrier mediated exchange diffusion could be demonstrated between [3H]GABA in preloaded rat cortical slices and unlabeled DABA and β‐alanine in the superfusion medium. These structurally related amino acids were chosen to investigate the source of releasable [3H]GABA because of evidence suggesting they may have differing affinities for the GABA carrier transport system that are specific for neurons and glia, DABA having a greater affinity for the neuronal GABA system and β‐alanine for the glial. Five millimolars‐DABA in the superfusion medium nearly abolished the K+ stimulated release of [3H]GABA whereas β‐alanine had little effect. The results and conclusions are discussed in terms of a postulated carrier mediated exchange of unlabeled DABA with a specific neuronal pool of [3H]GABA interfering with the K+ stimulated release of the radiolabeled GABA. The results provide indirect evidence in favor of a neuronal pool as the source of releasable [3H]GABA in this system.

Original languageEnglish (US)
Pages (from-to)399-403
Number of pages5
JournalJournal of neurochemistry
Volume27
Issue number2
DOIs
StatePublished - Aug 1976

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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