Abstract— The effect of L‐2,4 diaminobutyric acid (DABA) and β‐alanine on the K+ stimulated release of [3H]GABA was examined using a continuous superfusion system in which a carrier mediated exchange diffusion could be demonstrated between [3H]GABA in preloaded rat cortical slices and unlabeled DABA and β‐alanine in the superfusion medium. These structurally related amino acids were chosen to investigate the source of releasable [3H]GABA because of evidence suggesting they may have differing affinities for the GABA carrier transport system that are specific for neurons and glia, DABA having a greater affinity for the neuronal GABA system and β‐alanine for the glial. Five millimolars‐DABA in the superfusion medium nearly abolished the K+ stimulated release of [3H]GABA whereas β‐alanine had little effect. The results and conclusions are discussed in terms of a postulated carrier mediated exchange of unlabeled DABA with a specific neuronal pool of [3H]GABA interfering with the K+ stimulated release of the radiolabeled GABA. The results provide indirect evidence in favor of a neuronal pool as the source of releasable [3H]GABA in this system.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of neurochemistry|
|State||Published - Aug 1976|
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience