Release of [3H]GABA from rat cortical slices: neuronal vs glial origin

John Hammerstad, C. R. Lytle

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The effect of L 2,4 diaminobutyric acid (DABA) and β alanine on the K+ stimulated release of [3H]GABA was examined using a continuous superfusion system in which a carrier mediated exchange diffusion could be demonstrated between [3H]GABA in preloaded rat cortical slices and unlabeled DABA and β alanine in the superfusion medium. These structurally related amino acids were chosen to investigate the source of releasable [3H]GABA because of evidence suggesting they may have differing affinities for the GABA carrier transport system that are specific for neurons and glia, DABA having a greater affinity for the neuronal GABA system and β alanine for the glial. Five millimolars DABA in the superfusion medium nearly abolished the K+ stimulated release of [3H]GABA whereas β alanine had little effect. The results and conclusions are discussed in terms of a postulated carrier mediated exchange of unlabeled DABA with a specific neuronal pool of [3H]GABA interfering with the K+ stimulated release of the radiolabeled GABA. The results provide indirect evidence in favor of a neuronal pool as the source of releasable [3H]GABA in this system.

Original languageEnglish (US)
Pages (from-to)399-403
Number of pages5
JournalJournal of Neurochemistry
Volume27
Issue number2
StatePublished - 1976

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Neuroglia
gamma-Aminobutyric Acid
Rats
Alanine
Acids
Carrier transport
Neurons
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Release of [3H]GABA from rat cortical slices : neuronal vs glial origin. / Hammerstad, John; Lytle, C. R.

In: Journal of Neurochemistry, Vol. 27, No. 2, 1976, p. 399-403.

Research output: Contribution to journalArticle

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