Relaxin regulates fibrillin 2, but not fibrillin 1, mRNA and protein expression by human dermal fibroblasts and murine fetal skin

Chrishan S. Samuel, Lynn Y. Sakai, Edward P. Amento

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Relaxin modulates connective tissue remodeling by altering matrix molecule expression. We have found that relaxin specifically inhibits a microfibril component, fibrillin 2 (FBN2), without affecting fibrillin 1 (FBN1). Human dermal fibroblasts (HDFs) grown or stimulated to overexpress fibrillin expression were used to show that relaxin specifically down-regulated FBN2 mRNA and protein levels. Continuous exposure of HDFs to relaxin (30ng/ml) significantly (P<0.05) decreased fibrillin 2 protein (40%) while FBN1 protein expression was unchanged. Our in vitro studies were confirmed using relaxin null mice whereby the absence of relaxin was associated with increased FBN2 mRNA and protein in fetal skin from pregnant relaxin knockout mice. The regulation of FBN2 expression may be associated with functional changes in elastic tissues during development and growth.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume411
Issue number1
DOIs
StatePublished - Mar 1 2003

Keywords

  • Fetal murine skin
  • Fibrillin
  • Human dermal fibroblasts
  • Relaxin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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