TY - JOUR
T1 - RELAXATION OF VASCULAR SMOOTH MUSCLE INDUCED BY LOW‐POWER LASER RADIATION
AU - Chaudhry, Hina
AU - Lynch, Mary
AU - Schomacker, Kevin
AU - Birngruber, Reginald
AU - Gregory, Kenton
AU - Kochevar, Irene
PY - 1993/11
Y1 - 1993/11
N2 - The relaxation of rabbit aorta rings induced by low‐power laser radiation was investigated in vitro to determine the location of the chromophore(s) responsible for this response and evaluate possible mechanisms. An action spectrum for relaxation was measured on rabbit thoracic aorta rings precontracted with norepinephrine. The decrease in isometric tension was measured during exposure to laser light (351–625 nm) delivered via a fiber optic to a small spot on the adventitial surface. The shortest UV wavelength (351 nm) was 35‐fold more effective than 390 nm and 1700‐fold more effective than 460 nm. Ultraviolet wavelengths also produced greater maximum relaxation (0.40–0.45) than visible wavelengths (0.20–0.25), suggesting that photovasorelaxation involves more than one chromophore. The adventitial layer was not necessary for photovasorelaxation, indicating that the light is absorbed by a chromophore in the medial layer. The same degree of relaxation was obtained on rings without adventitia when either one‐half of the ring, or a small spot was irradiated indicating that communication between smooth muscle cells spreads a signal from the area illuminated to the entire ring. The mechanism for photovasorelaxation was investigated using potential inhibitors. N‐monomethyl‐l‐arginine and N‐amino‐L‐arginine, inhibitors of nitric oxide synthase, did not alter photovasorelaxation nor did indomethacin, an inhibitor of cyclooxygenase, and zinc protoporphyrin, an inhibitor of heme oxygenase.
AB - The relaxation of rabbit aorta rings induced by low‐power laser radiation was investigated in vitro to determine the location of the chromophore(s) responsible for this response and evaluate possible mechanisms. An action spectrum for relaxation was measured on rabbit thoracic aorta rings precontracted with norepinephrine. The decrease in isometric tension was measured during exposure to laser light (351–625 nm) delivered via a fiber optic to a small spot on the adventitial surface. The shortest UV wavelength (351 nm) was 35‐fold more effective than 390 nm and 1700‐fold more effective than 460 nm. Ultraviolet wavelengths also produced greater maximum relaxation (0.40–0.45) than visible wavelengths (0.20–0.25), suggesting that photovasorelaxation involves more than one chromophore. The adventitial layer was not necessary for photovasorelaxation, indicating that the light is absorbed by a chromophore in the medial layer. The same degree of relaxation was obtained on rings without adventitia when either one‐half of the ring, or a small spot was irradiated indicating that communication between smooth muscle cells spreads a signal from the area illuminated to the entire ring. The mechanism for photovasorelaxation was investigated using potential inhibitors. N‐monomethyl‐l‐arginine and N‐amino‐L‐arginine, inhibitors of nitric oxide synthase, did not alter photovasorelaxation nor did indomethacin, an inhibitor of cyclooxygenase, and zinc protoporphyrin, an inhibitor of heme oxygenase.
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U2 - 10.1111/j.1751-1097.1993.tb04949.x
DO - 10.1111/j.1751-1097.1993.tb04949.x
M3 - Article
C2 - 8284321
AN - SCOPUS:0027692248
SN - 0031-8655
VL - 58
SP - 661
EP - 669
JO - Photochemistry and photobiology
JF - Photochemistry and photobiology
IS - 5
ER -