Relaxation of vascular smooth muscle induced by low-power laser radiation.

H. Chaudhry, M. Lynch, K. Schomacker, R. Birngruber, Kenton Gregory, I. Kochevar

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The relaxation of rabbit aorta rings induced by low-power laser radiation was investigated in vitro to determine the location of the chromophore(s) responsible for this response and evaluate possible mechanisms. An action spectrum for relaxation was measured on rabbit thoracic aorta rings precontracted with norepinephrine. The decrease in isometric tension was measured during exposure to laser light (351-625 nm) delivered via a fiber optic to a small spot on the adventitial surface. The shortest UV wavelength (351 nm) was 35-fold more effective than 390 nm and 1700-fold more effective than 460 nm. Ultraviolet wavelengths also produced greater maximum relaxation (0.40-0.45) than visible wavelengths (0.20-0.25), suggesting that photovasorelaxation involves more than one chromophore. The adventitial layer was not necessary for photovasorelaxation, indicating that the light is absorbed by a chromophore in the medial layer. The same degree of relaxation was obtained on rings without adventitia when either one-half of the ring, or a small spot was irradiated indicating that communication between smooth muscle cells spreads a signal from the area illuminated to the entire ring. The mechanism for photovasorelaxation was investigated using potential inhibitors. N-monomethyl-L-arginine and N-amino-L-arginine, inhibitors of nitric oxide synthase, did not alter photovasorelaxation nor did indomethacin, an inhibitor of cyclooxygenase, and zinc protoporphyrin, an inhibitor of heme oxygenase.

Original languageEnglish (US)
Pages (from-to)661-669
Number of pages9
JournalPhotochemistry and Photobiology
Volume58
Issue number5
StatePublished - Nov 1993
Externally publishedYes

Fingerprint

smooth muscle
Adventitia
Laser radiation
Chromophores
Vascular Smooth Muscle
Muscle
Lasers
inhibitors
laser beams
Radiation
Wavelength
Arginine
rings
chromophores
aorta
Rabbits
rabbits
Light
Heme Oxygenase (Decyclizing)
Cyclooxygenase Inhibitors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Chaudhry, H., Lynch, M., Schomacker, K., Birngruber, R., Gregory, K., & Kochevar, I. (1993). Relaxation of vascular smooth muscle induced by low-power laser radiation. Photochemistry and Photobiology, 58(5), 661-669.

Relaxation of vascular smooth muscle induced by low-power laser radiation. / Chaudhry, H.; Lynch, M.; Schomacker, K.; Birngruber, R.; Gregory, Kenton; Kochevar, I.

In: Photochemistry and Photobiology, Vol. 58, No. 5, 11.1993, p. 661-669.

Research output: Contribution to journalArticle

Chaudhry, H, Lynch, M, Schomacker, K, Birngruber, R, Gregory, K & Kochevar, I 1993, 'Relaxation of vascular smooth muscle induced by low-power laser radiation.', Photochemistry and Photobiology, vol. 58, no. 5, pp. 661-669.
Chaudhry H, Lynch M, Schomacker K, Birngruber R, Gregory K, Kochevar I. Relaxation of vascular smooth muscle induced by low-power laser radiation. Photochemistry and Photobiology. 1993 Nov;58(5):661-669.
Chaudhry, H. ; Lynch, M. ; Schomacker, K. ; Birngruber, R. ; Gregory, Kenton ; Kochevar, I. / Relaxation of vascular smooth muscle induced by low-power laser radiation. In: Photochemistry and Photobiology. 1993 ; Vol. 58, No. 5. pp. 661-669.
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