Relationship of Friend murine leukemia virus production to growth and hemoglobin synthesis in cultured erythroleukemia cells

C. C. Sherton, L. H. Evans, E. Polonoff, David Kabat

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The factors that control oncornavirus formation were analyzed in Friend leukemia cells that undergo hematopoiesis when treated with dimethyl sulfoxide. Suspension cultures of Ostertag FSD 1 cell line were found to enter a G0 or resting state at the end of their proliferative phase and to simultaneously cease producing helper and dependent components of Friend virus. Whereas the decline in virus production is at least 100 fold, rates of cellular RNA and protein synthesis are only slightly lower in resting than in growing cells. Both resting and growing cells contain similarly large concentrations of the viral proteins P(30) and P(12). Dimethyl sulfoxide induces hemoglobin synthesis in growing cells, but its effects on virus production appear to be indirect results of its action to inhibit cell growth and thus to delay entry of cells into the G0 resting state. Furthermore, variant cell lines were obtained with differing abilities to synthesize virus or hemoglobin. Some lines no longer produce infectious virus, although they all harbor murine leukemia virus genes which are expressed to varying extents. The major internal protein of these oncornaviruses, P(30), is synthesized in large amounts by all of the cell lines. These results suggest that Friend virus production is not coinduced with erythroid differentiation, as had been proposed, but rather is controlled by the growth cycle of the cell.

Original languageEnglish (US)
Pages (from-to)118-125
Number of pages8
JournalJournal of Virology
Volume19
Issue number1
StatePublished - 1976

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Murine leukemia virus
Friend murine leukemia virus
Leukemia, Erythroblastic, Acute
cultured cells
Cultured Cells
hemoglobin
Hemoglobins
viruses
synthesis
Growth
Viruses
cell lines
Retroviridae
dimethyl sulfoxide
Dimethyl Sulfoxide
Cell Line
cells
Murine Leukemia Viruses
hematopoiesis
viral proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Relationship of Friend murine leukemia virus production to growth and hemoglobin synthesis in cultured erythroleukemia cells. / Sherton, C. C.; Evans, L. H.; Polonoff, E.; Kabat, David.

In: Journal of Virology, Vol. 19, No. 1, 1976, p. 118-125.

Research output: Contribution to journalArticle

Sherton, C. C. ; Evans, L. H. ; Polonoff, E. ; Kabat, David. / Relationship of Friend murine leukemia virus production to growth and hemoglobin synthesis in cultured erythroleukemia cells. In: Journal of Virology. 1976 ; Vol. 19, No. 1. pp. 118-125.
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