Relationship between increased cyclic ampphosphodiesterase activity and abnormal adenylyl cyclase regulation in leukocytes from patients with atopic dermatitis

Jon M. Hanifin, Rita Lloyd, Keiji Okubo, Larry L. Guerin, Linda Fancher, Sai C. Chan

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Atopic dermatitis (AD) is characterized by a variety of abnormal physiologic and pharmacologic responses in the skin. Leukocyte abnormalities of the cyclic nucleotide system include increased cAMP phosphodiesterase (PDE) and adenylyl cyclase activities. We have evaluated the possibility that a defect of the inhibitory GTP-binding protein (Gi) might cause inadequate modulation of adenylyl cyclase activity in AD leukocytes. We carried out a series of studies assessing adenylyl cyclase and Gi subunits in monocyte membranes. Using both pertussis toxin ribosylation and direct monoclonal antibody labeling of Gi proteins, we have shown evidence for a decrease or possible absence of one of the Gi proteins in atopic monocyte membranes. A genetic defect or toxin-mediated abnormality in leukocyte membrane Gi could account for these findings. Increased cAMP degradation by PDE may be a compensatory mechanism for increased cAMP synthesis that is regulated by GTP-binding proteins. But this increased PDE activity also rendered AD leukocytes hypo-responsive to immunofunction regulatory signals mediated by cAMP.

Original languageEnglish (US)
Pages (from-to)S100-S105
JournalJournal of Investigative Dermatology
Volume98
Issue number6 SUPPL.
DOIs
StatePublished - Jun 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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