TY - JOUR
T1 - Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation
AU - Barria, Andres
AU - Muller, Dominique
AU - Derkach, Victor
AU - Griffith, Leslie C.
AU - Soderling, Thomas R.
PY - 1997/6/27
Y1 - 1997/6/27
N2 - Long-term potentiation (LTP), a cellular model of learning and memory, requires calcium-dependent protein kinases. Induction of LTP increased the phosphorus-32 labeling of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPA-Rs), which mediate rapid excitatory synaptic transmission. This AMPA-R phosphorylation appeared to be catalyzed by Ca2+- and calmodulin-dependent protein kinase II (CaM-KII): (i) it correlated with the activation and autophosphorylation of CaM-KII, (ii) it was blocked by the CaM-KII inhibitor KN-62, and (iii) its phosphorus- 32 peptide map was the same as that of GluR1 coexpressed with activated CaM- KII in HEK-293 cells. This covalent modulation of AMPA-Rs in LTP provides a postsynaptic molecular mechanism for synaptic plasticity.
AB - Long-term potentiation (LTP), a cellular model of learning and memory, requires calcium-dependent protein kinases. Induction of LTP increased the phosphorus-32 labeling of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPA-Rs), which mediate rapid excitatory synaptic transmission. This AMPA-R phosphorylation appeared to be catalyzed by Ca2+- and calmodulin-dependent protein kinase II (CaM-KII): (i) it correlated with the activation and autophosphorylation of CaM-KII, (ii) it was blocked by the CaM-KII inhibitor KN-62, and (iii) its phosphorus- 32 peptide map was the same as that of GluR1 coexpressed with activated CaM- KII in HEK-293 cells. This covalent modulation of AMPA-Rs in LTP provides a postsynaptic molecular mechanism for synaptic plasticity.
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U2 - 10.1126/science.276.5321.2042
DO - 10.1126/science.276.5321.2042
M3 - Article
C2 - 9197267
AN - SCOPUS:0030744875
SN - 0036-8075
VL - 276
SP - 2042
EP - 2045
JO - Science
JF - Science
IS - 5321
ER -