Regulation of the PI3K pathway through a p85α monomer–homodimer equilibrium

Lydia W.T. Cheung, Katarzyna W. Walkiewicz, Tabot Md Besong, Huifang Guo, David H. Hawke, Stefan T. Arold, Gordon Mills

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The canonical action of the p85α regulatory subunit of phosphatidylinositol 3-kinase (PI3K) is to associate with the p110α catalytic subunit to allow stimuli-dependent activation of the PI3K pathway. We elucidate a p110α-independent role of homodimerized p85α in the positive regulation of PTEN stability and activity. p110α-free p85α homodimerizes via two intermolecular interactions (SH3:proline-rich region and BH:BH) to selectively bind unphosphorylated activated PTEN. As a consequence, homodimeric but not monomeric p85α suppresses the PI3K pathway by protecting PTEN from E3 ligase WWP2-mediated proteasomal degradation. Further, the p85α homodimer enhances the lipid phosphatase activity and membrane association of PTEN. Strikingly, we identified cancer patient-derived oncogenic p85α mutations that target the homodimerization or PTEN interaction surface. Collectively, our data suggest the equilibrium of p85α monomerdimers regulates the PI3K pathway and disrupting this equilibrium could lead to disease development.

Original languageEnglish (US)
Article numbere06866
JournaleLife
Volume4
Issue numberJULY 2015
DOIs
StatePublished - Jul 29 2015
Externally publishedYes

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Phosphatidylinositol 3-Kinase
Ubiquitin-Protein Ligases
Phosphoric Monoester Hydrolases
Proline
Catalytic Domain
Chemical activation
Association reactions
Membranes
Lipids
Degradation
Mutation
Neoplasms

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cheung, L. W. T., Walkiewicz, K. W., Besong, T. M., Guo, H., Hawke, D. H., Arold, S. T., & Mills, G. (2015). Regulation of the PI3K pathway through a p85α monomer–homodimer equilibrium. eLife, 4(JULY 2015), [e06866]. https://doi.org/10.7554/eLife.06866

Regulation of the PI3K pathway through a p85α monomer–homodimer equilibrium. / Cheung, Lydia W.T.; Walkiewicz, Katarzyna W.; Besong, Tabot Md; Guo, Huifang; Hawke, David H.; Arold, Stefan T.; Mills, Gordon.

In: eLife, Vol. 4, No. JULY 2015, e06866, 29.07.2015.

Research output: Contribution to journalArticle

Cheung, LWT, Walkiewicz, KW, Besong, TM, Guo, H, Hawke, DH, Arold, ST & Mills, G 2015, 'Regulation of the PI3K pathway through a p85α monomer–homodimer equilibrium', eLife, vol. 4, no. JULY 2015, e06866. https://doi.org/10.7554/eLife.06866
Cheung LWT, Walkiewicz KW, Besong TM, Guo H, Hawke DH, Arold ST et al. Regulation of the PI3K pathway through a p85α monomer–homodimer equilibrium. eLife. 2015 Jul 29;4(JULY 2015). e06866. https://doi.org/10.7554/eLife.06866
Cheung, Lydia W.T. ; Walkiewicz, Katarzyna W. ; Besong, Tabot Md ; Guo, Huifang ; Hawke, David H. ; Arold, Stefan T. ; Mills, Gordon. / Regulation of the PI3K pathway through a p85α monomer–homodimer equilibrium. In: eLife. 2015 ; Vol. 4, No. JULY 2015.
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