Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins

Judit Remenyi, Christopher J. Hunter, Christian Cole, Hideaki Ando, Soren Impey, Claire E. Monk, Kirsty J. Martin, Geoffrey J. Barton, Gyorgy Hutvagner, J. Simon C Arthur

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Neurotrophins are growth factors that are important in neuronal development and survival as well as synapse formation and plasticity. Many of the effects of neurotrophins are mediated by changes in protein expression as a result of altered transcription or translation. To determine whether neurotrophins regulate the production of microRNAs (miRNAs), small RNA species that modulate protein translation or mRNA stability, we used deep sequencing to identify BDNF (brain-derived neurotrophic factor)-induced miRNAs in cultured primary cortical mouse neurons. This revealed that the miR-212/132 cluster contained the miRNAs most responsive to BDNF treatment. This cluster was found to produce four miRNAs: miR-132, miR-132*, miR-212 and miR-212*. Using specific inhibitors, mouse models and promoter analysis we have shown that the regulation of the transcription of the miR-212/132 miRNA cluster and the miRNAs derived from it are regulated by the ERK1/2 (extracellular-signal-regulated kinase 1/2) pathway, via bothMSK (mitogen and stress-activated kinase)-dependent and -independent mechanisms.

Original languageEnglish (US)
Pages (from-to)281-291
Number of pages11
JournalBiochemical Journal
Volume428
Issue number2
DOIs
StatePublished - Jun 1 2010

Fingerprint

Nerve Growth Factors
MicroRNAs
Brain-Derived Neurotrophic Factor
Transcription
High-Throughput Nucleotide Sequencing
Mitogen-Activated Protein Kinase 3
Protein Stability
Mitogen-Activated Protein Kinase 1
RNA Stability
Protein Biosynthesis
Mitogens
Synapses
Neurons
Plasticity
Intercellular Signaling Peptides and Proteins
Proteins
Phosphotransferases
RNA
Messenger RNA

Keywords

  • Brain-derived neurotrophic factor (BDNF)
  • MicroRNA (miRNA)
  • Mitogen- and stress-activated kinase 1 (MSK1)
  • Mitogen- and stress-activated kinase 2 (MSK2)
  • Mitogen-activated protein kinase (MAPK)
  • Neuron

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Remenyi, J., Hunter, C. J., Cole, C., Ando, H., Impey, S., Monk, C. E., ... Arthur, J. S. C. (2010). Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins. Biochemical Journal, 428(2), 281-291. https://doi.org/10.1042/BJ20100024

Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins. / Remenyi, Judit; Hunter, Christopher J.; Cole, Christian; Ando, Hideaki; Impey, Soren; Monk, Claire E.; Martin, Kirsty J.; Barton, Geoffrey J.; Hutvagner, Gyorgy; Arthur, J. Simon C.

In: Biochemical Journal, Vol. 428, No. 2, 01.06.2010, p. 281-291.

Research output: Contribution to journalArticle

Remenyi, J, Hunter, CJ, Cole, C, Ando, H, Impey, S, Monk, CE, Martin, KJ, Barton, GJ, Hutvagner, G & Arthur, JSC 2010, 'Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins', Biochemical Journal, vol. 428, no. 2, pp. 281-291. https://doi.org/10.1042/BJ20100024
Remenyi, Judit ; Hunter, Christopher J. ; Cole, Christian ; Ando, Hideaki ; Impey, Soren ; Monk, Claire E. ; Martin, Kirsty J. ; Barton, Geoffrey J. ; Hutvagner, Gyorgy ; Arthur, J. Simon C. / Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins. In: Biochemical Journal. 2010 ; Vol. 428, No. 2. pp. 281-291.
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