Regulation of surface localization of the small conductance Ca 2+-activated potassium channel, Sk2, through direct phosphorylation by cAMP-dependent protein kinase

Yajun Ren, Lyndon F. Barnwell, Jon C. Alexander, Farah D. Lubin, John Adelman, Paul J. Pfaffinger, Laura A. Schrader, Anne E. Anderson

Research output: Contribution to journalArticle

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Abstract

Small conductance, Ca2+-activated voltage-independent potassium channels (SK channels) are widely expressed in diverse tissues; however, little is known about the molecular regulation of SK channel subunits. Direct alteration of ion channel subunits by kinases is a candidate mechanism for functional modulation of these channels. We find that activation of cyclic AMP-dependent protein kinase (PKA) with forskolin (50 μM) causes a dramatic decrease in surface localization of the SK2 channel subunit expressed in COS7 cells due to direct phosphorylation of the SK2 channel subunit.PKA phosphorylation studies using the intracellular domains of the SK2 channel subunit expressed as glutathione S-transferase fusion protein constructs showed that both the amino-terminal and carboxyl-terminal regions are PKA substrates in vitro. Mutational analysis identified a single PKA phosphorylation site within the amino-terminal of the SK2 subunit at serine 136. Mutagenesis and mass spectrometry studies identified four PKA phosphorylation sites: Ser 465 (minor site) and three amino acid residues Ser568, Ser569, and Ser570 (major sites) within the carboxyl-terminal region. A mutated SK2 channel subunit, with the three contiguous serines mutated to alanines to block phosphorylation at these sites, shows no decrease in surface expression after PKA stimulation. Thus, our findings suggest that PKA phosphorylation of these three sites is necessary for PKA-mediated reorganization of SK2 surface expression.

Original languageEnglish (US)
Pages (from-to)11769-11779
Number of pages11
JournalJournal of Biological Chemistry
Volume281
Issue number17
DOIs
StatePublished - Apr 28 2006

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Phosphorylation
Potassium Channels
Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Serine
Small-Conductance Calcium-Activated Potassium Channels
Mutagenesis
Colforsin
Glutathione Transferase
Ion Channels
Alanine
Mass spectrometry
Mass Spectrometry
Phosphotransferases
Fusion reactions
Chemical activation
Modulation
Tissue
Amino Acids
Electric potential

ASJC Scopus subject areas

  • Biochemistry

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Regulation of surface localization of the small conductance Ca 2+-activated potassium channel, Sk2, through direct phosphorylation by cAMP-dependent protein kinase. / Ren, Yajun; Barnwell, Lyndon F.; Alexander, Jon C.; Lubin, Farah D.; Adelman, John; Pfaffinger, Paul J.; Schrader, Laura A.; Anderson, Anne E.

In: Journal of Biological Chemistry, Vol. 281, No. 17, 28.04.2006, p. 11769-11779.

Research output: Contribution to journalArticle

Ren, Yajun ; Barnwell, Lyndon F. ; Alexander, Jon C. ; Lubin, Farah D. ; Adelman, John ; Pfaffinger, Paul J. ; Schrader, Laura A. ; Anderson, Anne E. / Regulation of surface localization of the small conductance Ca 2+-activated potassium channel, Sk2, through direct phosphorylation by cAMP-dependent protein kinase. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 17. pp. 11769-11779.
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