Regulation of rat brain/hepg2 glucose transporter gene expression by insulin and insulin-like growth factor-i in primary cultures of neuronal and glial cells

Haim Werner, Howard L. Foyt, Charles T. Roberts, Derek Leroith, Ian A. Simpson, Mohan K. Raizada, Laura M. Mudd

    Research output: Contribution to journalArticlepeer-review

    104 Scopus citations

    Abstract

    We have demonstrated the expression of the rat brain/HepG2 glucose transporter gene in primary cultures of rat neuronal and glial cells by Northern blot analysis with a rat brain glucose transporter cDNA probe. Incubation of both neuronal and glial cells with insulin and insulin-like growth factor- I induced a time- and dose-dependent increase in the steady state levels of glucose transporter mRNA. The maximal response was achieved between 2â€4 h and subsequently decreased. Both insulin and insulin-like growth factor-I at a dose of 1 ng/ml elicited an approximately 57% increase in glucose transporter mRNA levels in neuronal cultures after 90 min, suggesting that each peptide was acting through its own receptor. On the other hand, insulin stimulated [3H]2-deoxyglucose uptake in glial, but not neuronal, cells. These results suggest that insulin-like peptides regulate the expression of the rat brain/Hep G2 glucose transporter gene at both transcriptional and posttranscriptional levels, and that these regulatory mechanisms may be dissociated from each other. Insulin-like peptides may, therefore, participate in the control of brain energy metabolism.

    Original languageEnglish (US)
    Pages (from-to)314-320
    Number of pages7
    JournalEndocrinology
    Volume125
    Issue number1
    DOIs
    StatePublished - Jul 1989

    ASJC Scopus subject areas

    • Endocrinology

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