Regulation of Prostaglandin E2 synthesis in human amnion by protein kinase C

J. Sander, Leslie Myatt

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A role for protein kinase C (PKC) in mediation of prostaglandin E2 synthesis in human amnion cells has been suggested. We have investigated the specificity of the stimulation of PGE2 synthesis by phorbol esters and employed putative PKC inhibitors to demonstrate the specificity of PKC stimulation. The three phorbol esters, tetradecanoyl phorbol-13-acetate, phorbol-12,13-dibutyrate and phorbol-12,13-didecanoate gave concentration-dependent (10-10 - 10-7M) increases in PGE2 synthesis when added to aminon cells in monolayer, however, no effect was seen with the structurally similar phorbols phorbol-12-13-diacetate, 4α phorbol-12-13-didecanoate or phorbol base. The stimulatory effect of TPA (10-8M) on amnion PGE2 synthesis could be prevented by coincubation with the putative protein kinase C inhibitors 1-(5-isoquinoline sulphonyl) piperazine, 1-0-octadecyl-2-0-methyl-rac-glycero-3-phosphocholine, sphingosine and chlopromazine at concentrations of 10-6-10-4M. Addition of the transcription inhibitor actinomycin D at 10-6-10-5M prevented TPA (10-8M)-induced PGE2 synthesis. However, paradoxically, a further increase in PGE2 synthesis was seen when 10-9-10-7M actinomycin D was added together with TPA. The phospholipase A2 inhibitor quinacrine was able to prevent the TPA-induced increase in PGE2 synthesis even in the presense of exogenous arachidonic acid suggesting that phospholipase A2 may be a target for PKC action.

Original languageEnglish (US)
Pages (from-to)355-363
Number of pages9
JournalProstaglandins
Volume39
Issue number4
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Amnion
Dinoprostone
Protein Kinase C
Protein C Inhibitor
Dactinomycin
Phorbol Esters
Protein Kinase Inhibitors
Phorbols
Phorbol 12,13-Dibutyrate
Quinacrine
Sphingosine
Phosphorylcholine
Phospholipases A2
Transcription
Arachidonic Acid
Monolayers
Acetates

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Regulation of Prostaglandin E2 synthesis in human amnion by protein kinase C. / Sander, J.; Myatt, Leslie.

In: Prostaglandins, Vol. 39, No. 4, 1990, p. 355-363.

Research output: Contribution to journalArticle

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