Regulation of prolactin synthesis by estrogen

M. E. Lieberman, Richard Maurer, R. T. Stone, M. A. Shupnik, L. Vician, L. A. Baxter, R. Ryan, J. Gorski

Research output: Contribution to journalArticle

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Abstract

There is an increasing body of evidence implicating estradiol in the regulation of prolactin synthesis. We have recently demonstrated that treatment of rats with estradiol specifically increased the incorporation of precursors into prolactin and led to the accumulation of preprolactin mRNA. These effects of estradiol could be due either to a direct action on the pituitary or to an indirect effect mediated by the hypothalamus. To distinguish between these possibilities, we studied the response of dispersed rat pituitary cells maintained in culture. We found that cultured pituitary cells respond to estradiol by increased incorporation of precursors into prolactin but not into the bulk of other cellular proteins. The rate of increase in prolactin synthesis is dose dependent, reaching maximal levels in the physiological range of estradiol. At a concentration of 10-9M, estradiol, estriol and diethylstilbestrol are stimulatory whereas androgens, progesterone and cortisone have no significant effect. Exposure of cells to 10-8M, estradiol results in a 500% stimulation of prolactin synthesis after 7 days of culture. The time course and magnitude of response to estradiol in cultured pituitary cells is comparable to the response seen in vivo. Our findings indicate that estradiol can stimulate prolactin synthesis through a direct action on the pituitary.

Original languageEnglish (US)
Pages (from-to)21-36
Number of pages16
JournalAdvances in the biosciences
Volume25
Issue numberC
StatePublished - 1980
Externally publishedYes

Fingerprint

Prolactin
Estradiol
Estrogens
Cultured Cells
Estriol
Diethylstilbestrol
Cortisone
Androgens
Hypothalamus
Progesterone
Messenger RNA

Keywords

  • estradiol
  • pituitary
  • preprolactin
  • Prolactin
  • rat
  • sheep

Cite this

Lieberman, M. E., Maurer, R., Stone, R. T., Shupnik, M. A., Vician, L., Baxter, L. A., ... Gorski, J. (1980). Regulation of prolactin synthesis by estrogen. Advances in the biosciences, 25(C), 21-36.

Regulation of prolactin synthesis by estrogen. / Lieberman, M. E.; Maurer, Richard; Stone, R. T.; Shupnik, M. A.; Vician, L.; Baxter, L. A.; Ryan, R.; Gorski, J.

In: Advances in the biosciences, Vol. 25, No. C, 1980, p. 21-36.

Research output: Contribution to journalArticle

Lieberman, ME, Maurer, R, Stone, RT, Shupnik, MA, Vician, L, Baxter, LA, Ryan, R & Gorski, J 1980, 'Regulation of prolactin synthesis by estrogen', Advances in the biosciences, vol. 25, no. C, pp. 21-36.
Lieberman ME, Maurer R, Stone RT, Shupnik MA, Vician L, Baxter LA et al. Regulation of prolactin synthesis by estrogen. Advances in the biosciences. 1980;25(C):21-36.
Lieberman, M. E. ; Maurer, Richard ; Stone, R. T. ; Shupnik, M. A. ; Vician, L. ; Baxter, L. A. ; Ryan, R. ; Gorski, J. / Regulation of prolactin synthesis by estrogen. In: Advances in the biosciences. 1980 ; Vol. 25, No. C. pp. 21-36.
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