Regulation of progestin receptors in raphe neurons of steroid-treated monkeys

    Research output: Contribution to journalArticle

    68 Citations (Scopus)

    Abstract

    Progesterone increases prolactin secretion in estrogen-primed primates This action is probably mediated through a neural mechanism since lactotropes do not have progestin receptors (PR). This laboratory recently reported localization of PR in serotonin neurons of female macaques. Since serotonin (5HT) is a putative prolactin stimulatory agent, it was of interest to examine the regulation of PR in this neural system. Spayed monkeys were treated with either (1) an empty silastic capsule; (2) an estrogen (E)-filled capsule for 28 days, or (3) an E-filled capsule for 28 days supplemented with a progesterone (P)-filled capsule for the last 14 of the 28 days. Pontine tissue blocks were obtained at autopsy and processed for immunocytochemistry. Adjacent sections (10 μm) throughout the extent of the raphe nuclei were immunostained for 5HT and PR. 5HT-positive and PR-positive cells were counted in the same area of the dorsal and ventral raphe of adjacent sections at 4 representative levels and the PR/5HT ratio was calculated. The number of 5HT-positive cells was not different in spayed, E- or E+P-treated groups. E-treatment significantly increased the number of PR-positive cells and the PR/5HT ratio in the dorsal and ventral raphe. Supplementary P treatment did not significantly decrease the PR/5HT ratio in these areas. This data suggests that E induces PR in the 5HT neuronal system and that the expression of PR is maintained in the presence of chronically elevated progestin. Thus, the expression of PR in the raphe is consistent with the manner in which P increases prolactin secretion in estrogen-primed primates.

    Original languageEnglish (US)
    Pages (from-to)50-61
    Number of pages12
    JournalNeuroendocrinology
    Volume60
    Issue number1
    StatePublished - 1994

    Fingerprint

    Progesterone Receptors
    Haplorhini
    Steroids
    Neurons
    Capsules
    Prolactin
    Estrogens
    Primates
    Progesterone
    Serotonin
    Raphe Nuclei
    Macaca
    Progestins
    Autopsy
    Immunohistochemistry

    Keywords

    • Estrogen
    • Gonadal steroid receptors
    • Gonadal steroids
    • Macaque
    • Progesterone
    • Progestin receptors
    • Raphe
    • Serotonin

    ASJC Scopus subject areas

    • Endocrinology
    • Neuroscience(all)

    Cite this

    Regulation of progestin receptors in raphe neurons of steroid-treated monkeys. / Bethea, Cynthia.

    In: Neuroendocrinology, Vol. 60, No. 1, 1994, p. 50-61.

    Research output: Contribution to journalArticle

    @article{7a217df368754f1fb529bc0938cf97bf,
    title = "Regulation of progestin receptors in raphe neurons of steroid-treated monkeys",
    abstract = "Progesterone increases prolactin secretion in estrogen-primed primates This action is probably mediated through a neural mechanism since lactotropes do not have progestin receptors (PR). This laboratory recently reported localization of PR in serotonin neurons of female macaques. Since serotonin (5HT) is a putative prolactin stimulatory agent, it was of interest to examine the regulation of PR in this neural system. Spayed monkeys were treated with either (1) an empty silastic capsule; (2) an estrogen (E)-filled capsule for 28 days, or (3) an E-filled capsule for 28 days supplemented with a progesterone (P)-filled capsule for the last 14 of the 28 days. Pontine tissue blocks were obtained at autopsy and processed for immunocytochemistry. Adjacent sections (10 μm) throughout the extent of the raphe nuclei were immunostained for 5HT and PR. 5HT-positive and PR-positive cells were counted in the same area of the dorsal and ventral raphe of adjacent sections at 4 representative levels and the PR/5HT ratio was calculated. The number of 5HT-positive cells was not different in spayed, E- or E+P-treated groups. E-treatment significantly increased the number of PR-positive cells and the PR/5HT ratio in the dorsal and ventral raphe. Supplementary P treatment did not significantly decrease the PR/5HT ratio in these areas. This data suggests that E induces PR in the 5HT neuronal system and that the expression of PR is maintained in the presence of chronically elevated progestin. Thus, the expression of PR in the raphe is consistent with the manner in which P increases prolactin secretion in estrogen-primed primates.",
    keywords = "Estrogen, Gonadal steroid receptors, Gonadal steroids, Macaque, Progesterone, Progestin receptors, Raphe, Serotonin",
    author = "Cynthia Bethea",
    year = "1994",
    language = "English (US)",
    volume = "60",
    pages = "50--61",
    journal = "Neuroendocrinology",
    issn = "0028-3835",
    publisher = "S. Karger AG",
    number = "1",

    }

    TY - JOUR

    T1 - Regulation of progestin receptors in raphe neurons of steroid-treated monkeys

    AU - Bethea, Cynthia

    PY - 1994

    Y1 - 1994

    N2 - Progesterone increases prolactin secretion in estrogen-primed primates This action is probably mediated through a neural mechanism since lactotropes do not have progestin receptors (PR). This laboratory recently reported localization of PR in serotonin neurons of female macaques. Since serotonin (5HT) is a putative prolactin stimulatory agent, it was of interest to examine the regulation of PR in this neural system. Spayed monkeys were treated with either (1) an empty silastic capsule; (2) an estrogen (E)-filled capsule for 28 days, or (3) an E-filled capsule for 28 days supplemented with a progesterone (P)-filled capsule for the last 14 of the 28 days. Pontine tissue blocks were obtained at autopsy and processed for immunocytochemistry. Adjacent sections (10 μm) throughout the extent of the raphe nuclei were immunostained for 5HT and PR. 5HT-positive and PR-positive cells were counted in the same area of the dorsal and ventral raphe of adjacent sections at 4 representative levels and the PR/5HT ratio was calculated. The number of 5HT-positive cells was not different in spayed, E- or E+P-treated groups. E-treatment significantly increased the number of PR-positive cells and the PR/5HT ratio in the dorsal and ventral raphe. Supplementary P treatment did not significantly decrease the PR/5HT ratio in these areas. This data suggests that E induces PR in the 5HT neuronal system and that the expression of PR is maintained in the presence of chronically elevated progestin. Thus, the expression of PR in the raphe is consistent with the manner in which P increases prolactin secretion in estrogen-primed primates.

    AB - Progesterone increases prolactin secretion in estrogen-primed primates This action is probably mediated through a neural mechanism since lactotropes do not have progestin receptors (PR). This laboratory recently reported localization of PR in serotonin neurons of female macaques. Since serotonin (5HT) is a putative prolactin stimulatory agent, it was of interest to examine the regulation of PR in this neural system. Spayed monkeys were treated with either (1) an empty silastic capsule; (2) an estrogen (E)-filled capsule for 28 days, or (3) an E-filled capsule for 28 days supplemented with a progesterone (P)-filled capsule for the last 14 of the 28 days. Pontine tissue blocks were obtained at autopsy and processed for immunocytochemistry. Adjacent sections (10 μm) throughout the extent of the raphe nuclei were immunostained for 5HT and PR. 5HT-positive and PR-positive cells were counted in the same area of the dorsal and ventral raphe of adjacent sections at 4 representative levels and the PR/5HT ratio was calculated. The number of 5HT-positive cells was not different in spayed, E- or E+P-treated groups. E-treatment significantly increased the number of PR-positive cells and the PR/5HT ratio in the dorsal and ventral raphe. Supplementary P treatment did not significantly decrease the PR/5HT ratio in these areas. This data suggests that E induces PR in the 5HT neuronal system and that the expression of PR is maintained in the presence of chronically elevated progestin. Thus, the expression of PR in the raphe is consistent with the manner in which P increases prolactin secretion in estrogen-primed primates.

    KW - Estrogen

    KW - Gonadal steroid receptors

    KW - Gonadal steroids

    KW - Macaque

    KW - Progesterone

    KW - Progestin receptors

    KW - Raphe

    KW - Serotonin

    UR - http://www.scopus.com/inward/record.url?scp=0028339305&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0028339305&partnerID=8YFLogxK

    M3 - Article

    C2 - 8090282

    AN - SCOPUS:0028339305

    VL - 60

    SP - 50

    EP - 61

    JO - Neuroendocrinology

    JF - Neuroendocrinology

    SN - 0028-3835

    IS - 1

    ER -