Regulation of pro-opiomelanocortin gene transcription in individual cell nuclei

Robert T. Fremeau, James Lundblad, Dolan B. Pritchett, Josiah N. Wilcox, James L. Roberts

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

A nonrepetitive complementary RNA probe specific for an intervening sequence of the rat pro-opiomelanocortin (POMC) gene primary transcript was used to analyze the hormonal regulation of POMC gene transcription in individual cell nuclei in the rat pituitary by in situ hybridization. This probe recognized only full-length POMC heterogeneous nuclear RNA, as verified by Northern blots of pituitary RNA. When pituitary sections were hybridized with this 3H-labeled POMC intron A probe, silver grains were predominantly localized over the nuclei of cells that expressed POMC in the anterior and intermediate lobes. Adrenalectomy increased both the average grain density over corticotroph nuclei and the number of cells in the anterior pituitary with significant numbers of silver grains over their nucleus. Dexamethasone administration to intact or adrenalectomized rats results in the rapid (within 30 minutes) disappearance of silver grains over the nuclei of corticotrophs in the anterior lobe, suggesting that POMC gene transcription had been inhibited. However, adrenalectomy or dexamethasone administration did not alter the silver grain density over nuclei of intermediate lobe melanotrophs. Thus, this in situ hybridization assay utilizing an intervening sequence-specific POMC probe can measure rapid physiological changes in POMC heterogeneous nuclear RNA in individual cell nuclei.

Original languageEnglish (US)
Pages (from-to)1265-1269
Number of pages5
JournalScience
Volume234
Issue number4781
StatePublished - 1986
Externally publishedYes

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Pro-Opiomelanocortin
Cell Nucleus
Silver
Genes
Heterogeneous Nuclear RNA
Corticotrophs
Introns
Adrenalectomy
Dexamethasone
In Situ Hybridization
Melanotrophs
RNA Probes
Complementary RNA
Northern Blotting
Cell Count
RNA

ASJC Scopus subject areas

  • General

Cite this

Fremeau, R. T., Lundblad, J., Pritchett, D. B., Wilcox, J. N., & Roberts, J. L. (1986). Regulation of pro-opiomelanocortin gene transcription in individual cell nuclei. Science, 234(4781), 1265-1269.

Regulation of pro-opiomelanocortin gene transcription in individual cell nuclei. / Fremeau, Robert T.; Lundblad, James; Pritchett, Dolan B.; Wilcox, Josiah N.; Roberts, James L.

In: Science, Vol. 234, No. 4781, 1986, p. 1265-1269.

Research output: Contribution to journalArticle

Fremeau, RT, Lundblad, J, Pritchett, DB, Wilcox, JN & Roberts, JL 1986, 'Regulation of pro-opiomelanocortin gene transcription in individual cell nuclei', Science, vol. 234, no. 4781, pp. 1265-1269.
Fremeau RT, Lundblad J, Pritchett DB, Wilcox JN, Roberts JL. Regulation of pro-opiomelanocortin gene transcription in individual cell nuclei. Science. 1986;234(4781):1265-1269.
Fremeau, Robert T. ; Lundblad, James ; Pritchett, Dolan B. ; Wilcox, Josiah N. ; Roberts, James L. / Regulation of pro-opiomelanocortin gene transcription in individual cell nuclei. In: Science. 1986 ; Vol. 234, No. 4781. pp. 1265-1269.
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