Regulation of peptidergic vesicle mobility by secretagogues

C. L. Washburn, J. E. Bean, M. A. Silverman, M. J. Pellegrino, Phillip Yates, R. G. Allen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Neuropeptides are released into the extracellular space from large secretory granules. In order to reach their release sites, these granules are translocated on microtubules and thought to interact with filamentous actin as they approach the cell membrane. We have used a green fluorescent protein-tagged neuropeptide prohormone (prepro-orphanin FQ) to visualize vesicle trafficking dynamics in NS20Y cells and cultures of primary hippocampal neurons. We found that the majority of secretory granules were mobile and accumulated at both the tips of neurites as well as other apparently specialized cellular sites. We also used live-cell imaging to test the notion that peptidergic vesicle mobility was regulated by secretagogues. We show that treatment with forskolin appeared to increase vesicle rates of speed, while depolarization with high K+ had no effect, even though both treatments stimulated neuropeptide secretion. In cultured hippocampal neurons the green fluorescent protein-tagged secretory vesicles were routed to both dendrites and axons, indicating that peptidergic vesicle transport was not polarized. Basal peptidergic vesicle mobility rates in hippocampal neurons were the same as those in NS20Y cells. Taken together, these studies suggest that secretory vesicle mobility is regulated by specific classes of secretagogues and that neuropeptide containing secretory vesicles may be released from dendritic structures.

Original languageEnglish (US)
Pages (from-to)801-809
Number of pages9
JournalTraffic
Volume3
Issue number11
DOIs
StatePublished - Nov 1 2002

Fingerprint

Secretory Vesicles
Neuropeptides
Neurons
Green Fluorescent Proteins
Depolarization
Colforsin
Cell membranes
Transport Vesicles
Primary Cell Culture
Extracellular Space
Actins
Neurites
Dendrites
Microtubules
Axons
Imaging techniques
Cell Membrane

Keywords

  • GFP
  • Mobility
  • Motors
  • Peptidergic vesicles

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Washburn, C. L., Bean, J. E., Silverman, M. A., Pellegrino, M. J., Yates, P., & Allen, R. G. (2002). Regulation of peptidergic vesicle mobility by secretagogues. Traffic, 3(11), 801-809. https://doi.org/10.1034/j.1600-0854.2002.31105.x

Regulation of peptidergic vesicle mobility by secretagogues. / Washburn, C. L.; Bean, J. E.; Silverman, M. A.; Pellegrino, M. J.; Yates, Phillip; Allen, R. G.

In: Traffic, Vol. 3, No. 11, 01.11.2002, p. 801-809.

Research output: Contribution to journalArticle

Washburn, CL, Bean, JE, Silverman, MA, Pellegrino, MJ, Yates, P & Allen, RG 2002, 'Regulation of peptidergic vesicle mobility by secretagogues', Traffic, vol. 3, no. 11, pp. 801-809. https://doi.org/10.1034/j.1600-0854.2002.31105.x
Washburn CL, Bean JE, Silverman MA, Pellegrino MJ, Yates P, Allen RG. Regulation of peptidergic vesicle mobility by secretagogues. Traffic. 2002 Nov 1;3(11):801-809. https://doi.org/10.1034/j.1600-0854.2002.31105.x
Washburn, C. L. ; Bean, J. E. ; Silverman, M. A. ; Pellegrino, M. J. ; Yates, Phillip ; Allen, R. G. / Regulation of peptidergic vesicle mobility by secretagogues. In: Traffic. 2002 ; Vol. 3, No. 11. pp. 801-809.
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