Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos

    Research output: Chapter in Book/Report/Conference proceedingChapter

    3 Scopus citations

    Abstract

    Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages141-150
    Number of pages10
    DOIs
    StatePublished - 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1565
    ISSN (Print)1064-3745

    Keywords

    • Alternative polyadenylation
    • KCNH2
    • Long QT syndrome
    • Morpholino
    • Potassium channel
    • hERG

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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