Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos

Qiuming Gong, Zhengfeng Zhou

    Research output: Chapter in Book/Report/Conference proceeding › Chapter

    1 Citation (Scopus)

    Abstract

    Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages141-150
    Number of pages10
    Volume1565
    DOIs
    StatePublished - 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1565
    ISSN (Print)10643745

    Fingerprint

    Morpholinos
    Polyadenylation
    Protein Sorting Signals
    Protein Isoforms
    Gene Expression Regulation
    Up-Regulation
    Genes
    Therapeutics

    Keywords

    • Alternative polyadenylation
    • hERG
    • KCNH2
    • Long QT syndrome
    • Morpholino
    • Potassium channel

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

    Cite this

    Gong, Q., & Zhou, Z. (2017). Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos. In Methods in Molecular Biology (Vol. 1565, pp. 141-150). (Methods in Molecular Biology; Vol. 1565). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6817-6_12

    Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos. / Gong, Qiuming; Zhou, Zhengfeng.

    Methods in Molecular Biology. Vol. 1565 Humana Press Inc., 2017. p. 141-150 (Methods in Molecular Biology; Vol. 1565).

    Research output: Chapter in Book/Report/Conference proceeding › Chapter

    Gong, Q & Zhou, Z 2017, Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos. in Methods in Molecular Biology. vol. 1565, Methods in Molecular Biology, vol. 1565, Humana Press Inc., pp. 141-150. https://doi.org/10.1007/978-1-4939-6817-6_12
    Gong Q, Zhou Z. Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos. In Methods in Molecular Biology. Vol. 1565. Humana Press Inc. 2017. p. 141-150. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6817-6_12
    Gong, Qiuming ; Zhou, Zhengfeng. / Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos. Methods in Molecular Biology. Vol. 1565 Humana Press Inc., 2017. pp. 141-150 (Methods in Molecular Biology).
    @inbook{00926f17c12f48ae9d0bc5cf21320b1a,
    title = "Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos",
    abstract = "Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.",
    keywords = "Alternative polyadenylation, hERG, KCNH2, Long QT syndrome, Morpholino, Potassium channel",
    author = "Qiuming Gong and Zhengfeng Zhou",
    year = "2017",
    doi = "10.1007/978-1-4939-6817-6_12",
    language = "English (US)",
    volume = "1565",
    series = "Methods in Molecular Biology",
    publisher = "Humana Press Inc.",
    pages = "141--150",
    booktitle = "Methods in Molecular Biology",

    }

    TY - CHAP

    T1 - Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos

    AU - Gong, Qiuming

    AU - Zhou, Zhengfeng

    PY - 2017

    Y1 - 2017

    N2 - Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.

    AB - Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.

    KW - Alternative polyadenylation

    KW - hERG

    KW - KCNH2

    KW - Long QT syndrome

    KW - Morpholino

    KW - Potassium channel

    UR - http://www.scopus.com/inward/record.url?scp=85017031500&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85017031500&partnerID=8YFLogxK

    U2 - 10.1007/978-1-4939-6817-6_12

    DO - 10.1007/978-1-4939-6817-6_12

    M3 - Chapter

    VL - 1565

    T3 - Methods in Molecular Biology

    SP - 141

    EP - 150

    BT - Methods in Molecular Biology

    PB - Humana Press Inc.

    ER -

    Access to Document