Abstract
Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.
Original language | English (US) |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 141-150 |
Number of pages | 10 |
Volume | 1565 |
DOIs | |
State | Published - 2017 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 1565 |
ISSN (Print) | 10643745 |
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Keywords
- Alternative polyadenylation
- hERG
- KCNH2
- Long QT syndrome
- Morpholino
- Potassium channel
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos. / Gong, Qiuming; Zhou, Zhengfeng.
Methods in Molecular Biology. Vol. 1565 Humana Press Inc., 2017. p. 141-150 (Methods in Molecular Biology; Vol. 1565).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Regulation of isoform expression by blocking polyadenylation signal sequences with morpholinos
AU - Gong, Qiuming
AU - Zhou, Zhengfeng
PY - 2017
Y1 - 2017
N2 - Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.
AB - Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.
KW - Alternative polyadenylation
KW - hERG
KW - KCNH2
KW - Long QT syndrome
KW - Morpholino
KW - Potassium channel
UR - http://www.scopus.com/inward/record.url?scp=85017031500&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017031500&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6817-6_12
DO - 10.1007/978-1-4939-6817-6_12
M3 - Chapter
C2 - 28364240
AN - SCOPUS:85017031500
VL - 1565
T3 - Methods in Molecular Biology
SP - 141
EP - 150
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -