Regulation of growth hormone release from cultured human pituitary adenomas by somatomedins and insulin

G. P. Ceda, A. R. Hoffman, G. D. Silverberg, D. M. Wilson, Ronald (Ron) Rosenfeld

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

GH secretion is stimulated by hypothalamic GH-releasing factor (GHRH) and inhibited by somatostatin. Since GH induces the production of insulin-like growth factors (IGF) in liver and other tissues, it is of interest to learn whether IGF alters GH release through long loop feedback inhibition. Pituitary adenomas which had been removed from six acromegalic patients were processed for dispersed cell cultures and/or cell membrane preparations. Binding studies using 125I-labeled IGF-I, IGF-II, and insulin revealed specific hormone binding for each ligand to cell membranes derived from four somatotropinomas. A partially purified somatomedin preparation inhibited basal and/or GHRH-stimulated GH release from cultured pituitary cells derived from three of four adenomas; there was no effect of somatomedin in one tumor. In a single tumor, insulin also partially inhibited GHRH-stimulated GH release. Additionally, in one nonadenomatous pituitary removed from a patient with diabetes mellitus, insulin and somatomedin inhibited GHRH-stimulated GH release, and insulin inhibited basal GH secretion. These results indicate that specific cell membrane receptors for somatomedin peptides and insulin may be found on cell membranes from GH-secreting tumors, and that somatomedins and insulin can inhibit GH release in cultured human somatotropinoma cells. Thus, these data suggest that somatomedins may exert feedback inhibition of GH secretion in some patients with acromegaly.

Original languageEnglish (US)
Pages (from-to)1204-1209
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume60
Issue number6
StatePublished - 1985
Externally publishedYes

Fingerprint

Pituitary Neoplasms
Somatomedins
Growth Hormone
Insulin
Cell membranes
Cell Membrane
Tumors
Pituitary Hormone-Releasing Hormones
Somatomedin Receptors
Feedback
Neoplasms
Insulin-Like Growth Factor II
Acromegaly
Peptide Receptors
Insulin Receptor
Medical problems
Somatostatin
Insulin-Like Growth Factor I
Cell culture
Adenoma

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Ceda, G. P., Hoffman, A. R., Silverberg, G. D., Wilson, D. M., & Rosenfeld, R. R. (1985). Regulation of growth hormone release from cultured human pituitary adenomas by somatomedins and insulin. Journal of Clinical Endocrinology and Metabolism, 60(6), 1204-1209.

Regulation of growth hormone release from cultured human pituitary adenomas by somatomedins and insulin. / Ceda, G. P.; Hoffman, A. R.; Silverberg, G. D.; Wilson, D. M.; Rosenfeld, Ronald (Ron).

In: Journal of Clinical Endocrinology and Metabolism, Vol. 60, No. 6, 1985, p. 1204-1209.

Research output: Contribution to journalArticle

Ceda, GP, Hoffman, AR, Silverberg, GD, Wilson, DM & Rosenfeld, RR 1985, 'Regulation of growth hormone release from cultured human pituitary adenomas by somatomedins and insulin', Journal of Clinical Endocrinology and Metabolism, vol. 60, no. 6, pp. 1204-1209.
Ceda, G. P. ; Hoffman, A. R. ; Silverberg, G. D. ; Wilson, D. M. ; Rosenfeld, Ronald (Ron). / Regulation of growth hormone release from cultured human pituitary adenomas by somatomedins and insulin. In: Journal of Clinical Endocrinology and Metabolism. 1985 ; Vol. 60, No. 6. pp. 1204-1209.
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