Regulation of fructose 2,6-P2 concentration in isolated hepatocytes

Carolyn Sue Richards, Eisuke Furuya, Kosaku Uyeda

Research output: Contribution to journalArticle

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Abstract

The effect of hormones on fructose-2,6-P2 level and fructose-6-P,2-kinase activity was examined using rat hepatocytes. The dose response curve shows the half-maximal effect of glucagon on fructose-2,6-P2 occurs at 3 X 10-13 M glucagon, whereas the half-maximal effect on cyclic AMP occurs at 3 × 10-0 M. The decrease in fructose-2,6-P2 parallels the decrease in fructose-6-P,2-kinase activity. Incubation of cells with dibutryl cyclic AMP and cyclic AMP results in a 2- to 3-fold decrease in fructose-2,6-P2. Epinephrine (10-5 M) mediates a 2-fold decrease in fructose-2,6-P2; isoproterenol has no effect. These results suggest that regulation of fructose-6-P,2-kinase is complex, involving cyclic AMP-dependent and -independent mechanisms.

Original languageEnglish (US)
Pages (from-to)1673-1679
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume100
Issue number4
DOIs
StatePublished - Jun 30 1981

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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