Regulation of fructose 2,6-P2 concentration in isolated hepatocytes

Carolyn Sue Richards; Eisuke Furuya; Kosaku Uyeda

doi:10.1016/0006-291X(81)90711-7

Regulation of fructose 2,6-P₂ concentration in isolated hepatocytes

Carolyn Sue Richards, Eisuke Furuya, Kosaku Uyeda

Research output: Contribution to journal › Article › peer-review

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Abstract

The effect of hormones on fructose-2,6-P₂ level and fructose-6-P,2-kinase activity was examined using rat hepatocytes. The dose response curve shows the half-maximal effect of glucagon on fructose-2,6-P₂ occurs at 3 X 10^-13 M glucagon, whereas the half-maximal effect on cyclic AMP occurs at 3 × 10^-0 M. The decrease in fructose-2,6-P₂ parallels the decrease in fructose-6-P,2-kinase activity. Incubation of cells with dibutryl cyclic AMP and cyclic AMP results in a 2- to 3-fold decrease in fructose-2,6-P₂. Epinephrine (10^-5 M) mediates a 2-fold decrease in fructose-2,6-P₂; isoproterenol has no effect. These results suggest that regulation of fructose-6-P,2-kinase is complex, involving cyclic AMP-dependent and -independent mechanisms.

Original language	English (US)
Pages (from-to)	1673-1679
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	100
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(81)90711-7
State	Published - Jun 30 1981
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Regulation of fructose 2,6-P2 concentration in isolated hepatocytes",

abstract = "The effect of hormones on fructose-2,6-P2 level and fructose-6-P,2-kinase activity was examined using rat hepatocytes. The dose response curve shows the half-maximal effect of glucagon on fructose-2,6-P2 occurs at 3 X 10-13 M glucagon, whereas the half-maximal effect on cyclic AMP occurs at 3 × 10-0 M. The decrease in fructose-2,6-P2 parallels the decrease in fructose-6-P,2-kinase activity. Incubation of cells with dibutryl cyclic AMP and cyclic AMP results in a 2- to 3-fold decrease in fructose-2,6-P2. Epinephrine (10-5 M) mediates a 2-fold decrease in fructose-2,6-P2; isoproterenol has no effect. These results suggest that regulation of fructose-6-P,2-kinase is complex, involving cyclic AMP-dependent and -independent mechanisms.",

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AU - Furuya, Eisuke

AU - Uyeda, Kosaku

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N2 - The effect of hormones on fructose-2,6-P2 level and fructose-6-P,2-kinase activity was examined using rat hepatocytes. The dose response curve shows the half-maximal effect of glucagon on fructose-2,6-P2 occurs at 3 X 10-13 M glucagon, whereas the half-maximal effect on cyclic AMP occurs at 3 × 10-0 M. The decrease in fructose-2,6-P2 parallels the decrease in fructose-6-P,2-kinase activity. Incubation of cells with dibutryl cyclic AMP and cyclic AMP results in a 2- to 3-fold decrease in fructose-2,6-P2. Epinephrine (10-5 M) mediates a 2-fold decrease in fructose-2,6-P2; isoproterenol has no effect. These results suggest that regulation of fructose-6-P,2-kinase is complex, involving cyclic AMP-dependent and -independent mechanisms.

AB - The effect of hormones on fructose-2,6-P2 level and fructose-6-P,2-kinase activity was examined using rat hepatocytes. The dose response curve shows the half-maximal effect of glucagon on fructose-2,6-P2 occurs at 3 X 10-13 M glucagon, whereas the half-maximal effect on cyclic AMP occurs at 3 × 10-0 M. The decrease in fructose-2,6-P2 parallels the decrease in fructose-6-P,2-kinase activity. Incubation of cells with dibutryl cyclic AMP and cyclic AMP results in a 2- to 3-fold decrease in fructose-2,6-P2. Epinephrine (10-5 M) mediates a 2-fold decrease in fructose-2,6-P2; isoproterenol has no effect. These results suggest that regulation of fructose-6-P,2-kinase is complex, involving cyclic AMP-dependent and -independent mechanisms.

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Regulation of fructose 2,6-P₂ concentration in isolated hepatocytes

Abstract

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