Regulation of fructose 2,6-P2 concentration in isolated hepatocytes

Carolyn (Sue) Richards, Eisuke Furuya, Kosaku Uyeda

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The effect of hormones on fructose-2,6-P2 level and fructose-6-P,2-kinase activity was examined using rat hepatocytes. The dose response curve shows the half-maximal effect of glucagon on fructose-2,6-P2 occurs at 3 X 10-13 M glucagon, whereas the half-maximal effect on cyclic AMP occurs at 3 × 10-0 M. The decrease in fructose-2,6-P2 parallels the decrease in fructose-6-P,2-kinase activity. Incubation of cells with dibutryl cyclic AMP and cyclic AMP results in a 2- to 3-fold decrease in fructose-2,6-P2. Epinephrine (10-5 M) mediates a 2-fold decrease in fructose-2,6-P2; isoproterenol has no effect. These results suggest that regulation of fructose-6-P,2-kinase is complex, involving cyclic AMP-dependent and -independent mechanisms.

Original languageEnglish (US)
Pages (from-to)1673-1679
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume100
Issue number4
DOIs
StatePublished - Jun 30 1981
Externally publishedYes

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Fructose
Phosphofructokinase-2
Hepatocytes
Cyclic AMP
Glucagon
Isoproterenol
Epinephrine
Rats
Cells
Hormones

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Regulation of fructose 2,6-P2 concentration in isolated hepatocytes. / Richards, Carolyn (Sue); Furuya, Eisuke; Uyeda, Kosaku.

In: Biochemical and Biophysical Research Communications, Vol. 100, No. 4, 30.06.1981, p. 1673-1679.

Research output: Contribution to journalArticle

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