Regulation of expression of A-kinase anchoring proteins in rat granulosa cells

Mary Hunzicker-Dunn, John D. Scott, Daniel Carr

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

FSH action on granulosa cells involves the generation of cAMP and subsequent activation of the cAMP-dependent protein kinase (PKA). The PKA holoenzyme is targeted to specific subcellular sites through the interaction of the regulatory subunits with A-kinase anchoring proteins (AKAPs). We previously reported that FSH regulates expression of AKAPs. In this report we examine the relationship between AKAP expression and cell shape. Granulosa cells cultured in the absence of FSH tend to spread and flatten. Cell spreading is accompanied by an increased expression of a 140-kDa AKAP. This spreading/flattening phenotype is independent of the specific extracellular matrix proteins (fibronectin, polylysine, and gelatin) on which cells are plated. Addition of FSH prevents both cell spreading and induction of AKAP 140. Culturing cells on poly (2-hydroxyethyl methacrylate), a surface- coating agent that inhibits cell spreading and adhesion, also inhibits expression of AKAP 140. Addition of phorbol myristate acetate, an agent known to antagonize FSH actions, blocks FSH regulation of both cell shape and AKAP 140 expression. Addition of dexamethasone plus FSH causes a synergistic increase in progesterone levels but has no effect on cell shape or induction of AKAP 140. Dexamethasone produces a dose-dependent increase in AKAP 80 expression, which is blocked by FSH, suggesting cross talk between the glucocorticoid and FSH receptor signaling pathways. These data suggest that expression of AKAP 140 is linked to regulation of cell shape, and that changes in the expression of AKAPs are regulated by several different signaling pathways.

Original languageEnglish (US)
Pages (from-to)1496-1502
Number of pages7
JournalBiology of Reproduction
Volume58
Issue number6
DOIs
StatePublished - Jun 1998

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Granulosa Cells
Protein Kinases
Cell Shape
Dexamethasone
FSH Receptors
Holoenzymes
Polylysine
Extracellular Matrix Proteins
Glucocorticoid Receptors
Tetradecanoylphorbol Acetate
Gelatin
Cyclic AMP-Dependent Protein Kinases
Fibronectins
Cell Adhesion
Progesterone

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Regulation of expression of A-kinase anchoring proteins in rat granulosa cells. / Hunzicker-Dunn, Mary; Scott, John D.; Carr, Daniel.

In: Biology of Reproduction, Vol. 58, No. 6, 06.1998, p. 1496-1502.

Research output: Contribution to journalArticle

Hunzicker-Dunn, Mary ; Scott, John D. ; Carr, Daniel. / Regulation of expression of A-kinase anchoring proteins in rat granulosa cells. In: Biology of Reproduction. 1998 ; Vol. 58, No. 6. pp. 1496-1502.
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