Regulation of ciaXRH operon expression and identification of the CiaR regulon in Streptococcus mutans

Chenggang Wu, Eduardo A. Ayala, Jennifer S. Downey, Justin Merritt, Steven D. Goodman, Fengxia Qi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The ciaRH operon in Streptococcus mutans contains 3 contiguous genes, ciaXRH. Unlike the CiaRH system in other streptococci, only the ciaH-null mutant displays defective phenotypes, while the ciaR-null mutant behaves like the wild type. The objective of this study was to determine the mechanism of this unusual property. We demonstrate that the ciaH mutation caused a >20-fold increase in ciaR transcript synthesis. A ciaRH double deletion reversed the ciaH phenotype, suggesting that overexpressed ciaR might be responsible for the observed ciaH phenotypes. When ciaR was forced to be overexpressed by a transcriptional fusion to the ldh promoter in the wild-type background, the same ciaH phenotypes were restored, confirming the involvement of overexpressed ciaR in the ciaH phenotypes. The ciaH mutation and ciaR overexpression also caused transcriptional alterations in 100 genes, with 15 genes upregulated >5-fold. Bioinformatics analysis identified a putative CiaR regulon consisting of 8 genes/operons, including the ciaXRH operon itself, all of which were upregulated. In vitro footprinting on 4 of the 8 promoters revealed a protected region of 26 to 28 bp encompassing two direct repeats, NTTAAG-n5-WTTAAG, 10 bp upstream of the -10 region, indicating direct binding of the CiaR protein to these promoters. Taken together, we conclude that overexpressed CiaR, as a result of either ciaH deletion or forced expression from a constitutive promoter, is a mediator in the CiaH-regulated phenotypes.

Original languageEnglish (US)
Pages (from-to)4669-4679
Number of pages11
JournalJournal of Bacteriology
Volume192
Issue number18
DOIs
StatePublished - Sep 2010
Externally publishedYes

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Regulon
Streptococcus mutans
Operon
Phenotype
Genes
Mutation
Nucleic Acid Repetitive Sequences
Streptococcus
Computational Biology
Carrier Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Regulation of ciaXRH operon expression and identification of the CiaR regulon in Streptococcus mutans. / Wu, Chenggang; Ayala, Eduardo A.; Downey, Jennifer S.; Merritt, Justin; Goodman, Steven D.; Qi, Fengxia.

In: Journal of Bacteriology, Vol. 192, No. 18, 09.2010, p. 4669-4679.

Research output: Contribution to journalArticle

Wu, Chenggang ; Ayala, Eduardo A. ; Downey, Jennifer S. ; Merritt, Justin ; Goodman, Steven D. ; Qi, Fengxia. / Regulation of ciaXRH operon expression and identification of the CiaR regulon in Streptococcus mutans. In: Journal of Bacteriology. 2010 ; Vol. 192, No. 18. pp. 4669-4679.
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