Regulation of Chimeric Phosphoenolpyruvate Carboxykinase Genes by the trans-Dominant Locus TSE1

Mathew (Matt) Thayer, T. G. Lugo, R. J. Leach, R. E K Fournier

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Extinction of phosphoenolpyruvate carboxykinase (PCK) gene expression in hepatoma x fibroblast hybrids is mediated by a trans-acting genetic locus designated tissue-specific extinguisher 1 (TSE1). To identify PCK gene sequences required for extinction, hepatoma transfectants expressing PCK-thymidine kinase (TK) chimeric genes were fused with TK- fibroblasts and PCK-TK expression in the resulting hybrids was monitored. Expression of a PCK-TK chimera containing PCK sequences between base pairs -548 and +73 was extinguished in four of five hepatoma transfectants tested, although hybrids derived from one transfectant clone failed to extinguish PCK-TK expression. In contrast, crosses between hepatoma transfectants expressing the herpesvirus TK gene from its own promoter and TK- fibbroblasts produced TK+ hybrids; extinction of the transfected TK gene was not observed. Thus, rat PCK gene sequences between base pairs -548 and +73 are sufficient for tissue-specific extinction in hybrid cells. Extinction of PCK-TK gene expression in transfectant microcell hybrids mapped specifically to human chromosome 17, the site of human TSE1.

Original languageEnglish (US)
Pages (from-to)2660-2668
Number of pages9
JournalMolecular and Cellular Biology
Volume10
Issue number6
StatePublished - Jun 1990
Externally publishedYes

Fingerprint

Dominant Genes
Phosphoenolpyruvate
Thymidine Kinase
Hepatocellular Carcinoma
Genes
Base Pairing
Fibroblasts
Gene Expression
Chromosomes, Human, Pair 17
Genetic Loci
Hybrid Cells
Herpesviridae
Human Chromosomes
Clone Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Regulation of Chimeric Phosphoenolpyruvate Carboxykinase Genes by the trans-Dominant Locus TSE1. / Thayer, Mathew (Matt); Lugo, T. G.; Leach, R. J.; Fournier, R. E K.

In: Molecular and Cellular Biology, Vol. 10, No. 6, 06.1990, p. 2660-2668.

Research output: Contribution to journalArticle

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