Regulation of Chimeric Phosphoenolpyruvate Carboxykinase Genes by the trans-Dominant Locus TSE1

M. J. Thayer, T. G. Lugo, R. J. Leach, R. E.K. Fournier

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Extinction of phosphoenolpyruvate carboxykinase (PCK) gene expression in hepatoma x fibroblast hybrids is mediated by a trans-acting genetic locus designated tissue-specific extinguisher 1 (TSE1). To identify PCK gene sequences required for extinction, hepatoma transfectants expressing PCK-thymidine kinase (TK) chimeric genes were fused with TK- fibroblasts and PCK-TK expression in the resulting hybrids was monitored. Expression of a PCK-TK chimera containing PCK sequences between base pairs -548 and +73 was extinguished in four of five hepatoma transfectants tested, although hybrids derived from one transfectant clone failed to extinguish PCK-TK expression. In contrast, crosses between hepatoma transfectants expressing the herpesvirus TK gene from its own promoter and TK- fibbroblasts produced TK+ hybrids; extinction of the transfected TK gene was not observed. Thus, rat PCK gene sequences between base pairs -548 and +73 are sufficient for tissue-specific extinction in hybrid cells. Extinction of PCK-TK gene expression in transfectant microcell hybrids mapped specifically to human chromosome 17, the site of human TSE1.

Original languageEnglish (US)
Pages (from-to)2660-2668
Number of pages9
JournalMolecular and cellular biology
Volume10
Issue number6
DOIs
StatePublished - Jun 1990

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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