Regulation of Calcium Oscillations in β-Cells by Co-activated Cannabinoid Receptors

Aurélien Laguerre, Kaya Keutler, Sebastian Hauke, Carsten Schultz

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Pharmacological treatment of pancreatic β cells targeting cannabinoid receptors 1 and 2 (CB1 and CB2) has been shown to result in significant effects on insulin release, possibly by modulating intracellular calcium levels ([Ca2+]i). It is unclear how the interplay of CB1 and CB2 affects insulin secretion. Here, we demonstrate by the use of highly specific receptor antagonists and the recently developed photo-releasable endocannabinoid 2-arachidonoylglycerol that both receptors have counteracting effects on cytosolic calcium oscillations. We further show that both receptors are juxtaposed in a way that increases [Ca2+]i oscillations in silent β cells but dampens them in active ones. This study highlights a functional role of CB1 and CB2 acting in concert as a compensator/attenuator switch for regulating β cell excitability.

Original languageEnglish (US)
Pages (from-to)88-96.e3
JournalCell Chemical Biology
Volume28
Issue number1
DOIs
StatePublished - Jan 21 2021

Keywords

  • 2-AG
  • caged endocannabinoid
  • calcium signaling
  • cannabinoid receptors
  • fluorescence
  • insulin secretion
  • photolysis
  • β-cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'Regulation of Calcium Oscillations in β-Cells by Co-activated Cannabinoid Receptors'. Together they form a unique fingerprint.

Cite this