Regulation of BAD phosphorylation at serine 112 by the Ras-mitogen-activated protein kinase pathway

Xianjun Fang, Shuangxing Yu, Astrid Eder, Muling Mao, Robert C. Bast, Douglas Boyd, Gordon B. Mills

    Research output: Contribution to journalArticle

    214 Scopus citations

    Abstract

    The function of the pro-apoptotic molecule BAD is regulated by phosphorylation of two sites, serine-112 (Ser-112) and serine-136 (Ser-136). Phosphorylation at either site results in loss of the ability of BAD to heterodimerize with the survival proteins BCL-X(L) or BCL-2. Phosphorylated BAD binds to 14-3-3 and is sequestered in the cytoplasm. It has been shown that phosphorylation of BAD at Ser-136 is mediated by the serine/threonine protein kinase Akt-1/PKB which is downstream of phosphatidylinositol 3-kinase (PI3K). The signaling process leading to phosphorylation of BAD at Ser-112 has not been identified. In this study, we show that phosphorylation of the two serine residues of BAD is differentially regulated. While Ser-136 phosphorylation is concordant with activation of Akt, Ser-112 phosphorylation does not correlate with Akt activation. Instead, we demonstrate that activated Ras and Raf, which are upstream of mitogen-activated protein kinases (MAPK), stimulate selective phosphorylation of BAD at Ser-112. Furthermore, phosphorylation of Ser-112, but not Ser-136 requires activation of the MAPK pathway as the MEK inhibitor, PD 98059, blocks EGF-, as well as activated Ras- or Raf-mediated phosphorylation of BAD at Ser-112. Therefore, the PI3K-Akt and Ras-MAPK pathways converge at BAD by mediating phosphorylation of distinct serine residues.

    Original languageEnglish (US)
    Pages (from-to)6635-6640
    Number of pages6
    JournalOncogene
    Volume18
    Issue number48
    DOIs
    StatePublished - Nov 18 1999

    Keywords

    • Akt
    • Apoptosis
    • BAD
    • MAPK
    • Phosphorylation
    • Ras

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics
    • Cancer Research

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